Xie, Mengying; Wang, Zhiyi; Wan, Xinlong; Weng, Jie; Tu, Mengyun; Mei, Jin; Wang, Zhibin; Du, Xiaohong; Wang, Liangxing; Chen, Chan published their research in Journal of Biomaterials Applications on February 29 ,2020. The article was titled 《Crosslinking effects of branched PEG on decellularized lungs of rats for tissue engineering》.Recommanded Product: 76931-93-6 The article contains the following contents:
Poly (ethylene glycol) (PEG) has been paid much attention to its applications in tissue engineering. However, the studies on poly (ethylene glycol) in tissue applications were currently far from enough. To investigate the crosslinking effects of poly (ethylene glycol) in mech. properties, anti-enzymic ability and cytocompatibility, in this study, the poly (ethylene glycol) was crosslinked to decellularized lung scaffolds from rats. In order to obtain the PEGylated decellularized lung scaffold, the N-succinimidyl S-acetylthioacetate was first used to modify the decellularized lung scaffold, and a four-arm- poly (ethylene glycol) containing four acrylate groups was then crosslinked to the decellularized lung scaffold by the Michael addition reaction between acrylate group and sulfhydryl group. The results showed that the optimal concentration of poly (ethylene glycol) and reaction time of PEGylation of decellularized lung scaffold was 40 mg/mL and 4 h, resp. Histol. examinations, SEM and quantification of tissue morphol. by septal thickness consistently indicated no differences between PEGylated and normal decellularized lung scaffold in morphol. Compared with native lung and normal decellularized lung scaffold, the Young’s modulus and stiffness of PEGylated decellularized lung scaffold increased significantly, whereas enzymic degradation rate of PEGylated decellularized lung scaffold decreased significantly, suggesting that poly (ethylene glycol) significantly enhanced the biomech. property and anti-enzymic stability of decellularized lung scaffold. Further, no significant differences in cell viability were found between PEGylated and normal decellularized lung scaffold, suggesting no toxicity introduction of poly (ethylene glycol) into decellularized lung scaffold by the crosslinking. These findings may provide useful information for further applications of poly (ethylene glycol) in tissue engineering.2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate(cas: 76931-93-6Recommanded Product: 76931-93-6) was used in this study.
2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate(cas: 76931-93-6) belongs to pyrrolidine. Pyrrolidine being a good nucleophile easily undergoes electrophilic substitution reactions with different electrophiles such alkyl halides and acyl halides, and forms N-substituted pyrrolidines. N-Alkylpyrrolidine on further reaction with alkyl halide provided quaternary salts.Recommanded Product: 76931-93-6
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