Selective Zinc Sensor Molecules with Various Affinities for Zn2+, Revealing Dynamics and Regional Distribution of Synaptically Released Zn2+ in Hippocampal Slices was written by Komatsu, Kensuke;Kikuchi, Kazuya;Kojima, Hirotatsu;Urano, Yasuteru;Nagano, Tetsuo. And the article was included in Journal of the American Chemical Society in 2005.Synthetic Route of C7H9NO This article mentions the following:
The authors have developed a series of fluorescent Zn2+ sensor mols. with distinct affinities for Zn2+, because biol. Zn2+ concentrations vary over a wide range from sub-nanomolar to millimolar. The new sensors have Kd values in the range of 10-8-10-4 M, compared with 2.7 nM for ZnAF-2. They do not fluoresce in the presence of other biol. important metal ions such as calcium or magnesium, and they can detect Zn2+ within 100 ms. In cultured cells, the fluorescence intensity of ZnAF-2 was saturated at low Zn2+ concentration, while that of ZnAF-3 (Kd = 0.79 渭M) was not saturated even at relatively high Zn2+ concentrations In hippocampal slices, the authors measured synaptic release of Zn2+ in response to high-potassium-induced depolarization. ZnAF-2 showed similar levels of fluorescence increase in dentate gyrus (DG), CA3 and CA1, which were indistinguishable. However, ZnAF-3 showed a fluorescence increase only in DG. Thus, by using a combination of sensor mols., it was demonstrated for the first time that a higher Zn2+ concentration is released in DG than in CA3 or CA1 and that the authors can easily visualize Zn2+ concentration over a wide range. The authors believe that the use of various combinations of ZnAF family members will offer unprecedented versatility for fluorescence-microscopic imaging of Zn2+ in biol. applications. In the experiment, the researchers used many compounds, for example, 6-Methyl-2-pyridinemethanol (cas: 1122-71-0Synthetic Route of C7H9NO).
6-Methyl-2-pyridinemethanol (cas: 1122-71-0) belongs to alcohols. The oxygen atom of the strongly polarized O鈥旽 bond of an alcohol pulls electron density away from the hydrogen atom. This polarized hydrogen, which bears a partial positive charge, can form a hydrogen bond with a pair of nonbonding electrons on another oxygen atom. The most common reactions of alcohols can be classified as oxidation, dehydration, substitution, esterification, and reactions of alkoxides.Synthetic Route of C7H9NO
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts