Longitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product quality was written by Jimenez, Angela C.;Heist, Christopher A.;Navaei, Milad;Yeago, Carolyn;Roy, Krishnendu. And the article was included in Cytotherapy in 2022.Recommanded Product: 3391-86-4 This article mentions the following:
Cell therapies have emerged as a potentially transformative therapeutic modality in many chronic and incurable diseases. However, inherent donor and patient variabilities, complex manufacturing processes, lack of well-defined critical quality attributes and unavailability of in-line or at-line process or product anal. technologies result in significant variance in cell product quality and clin. trial outcomes. New approaches for overcoming these challenges are needed to realize the potential of cell therapies. Here the authors developed an untargeted two-dimensional gas chromatog. mass spectrometry-based method for non-destructive longitudinal at-line monitoring of cells during manufacturing to discover correlative volatile biomarkers of cell proliferation and end product potency. Specifically, using mesenchymal stromal cell cultures as a model, the authors demonstrated that GCxGC-MS of the culture medium headspace can effectively discriminate between media types and tissue sources. Headspace GCxGC-MS identified specific volatile compounds that showed a strong correlation with cell expansion and product functionality quantified by indoleamine-2,3-dioxygenase and T-cell proliferation/suppression assays. Addnl., the authors discovered increases in specific volatile metabolites when cells were treated with inflammatory stimulation. This work establishes GCxGC-MS as an at-line process anal. technol. for cell manufacturing that could improve culture robustness and may be used to non-destructively monitor culture state and correlate with end product function. In the experiment, the researchers used many compounds, for example, Oct-1-en-3-ol (cas: 3391-86-4Recommanded Product: 3391-86-4).
Oct-1-en-3-ol (cas: 3391-86-4) belongs to alcohols. Alcohols are weak acids. The most acidic simple alcohols (methanol and ethanol) are about as acidic as water, and most other alcohols are somewhat less acidic. Secondary alcohols are easily oxidized without breaking carbon-carbon bonds only as far as the ketone stage. No further oxidation is seen except under very stringent conditions.Recommanded Product: 3391-86-4
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts