Zhang, H.-L. published the artcileEnantioselective synthesis of enantiopure chiral alcohols using carbonyl reductases screened from Yarrowia lipolytica, Recommanded Product: 7-Chloro-2,3,4,5-tetrahydro-1H-benzo[b]azepin-5-ol, the publication is Journal of Applied Microbiology (2019), 126(1), 127-137, database is CAplus and MEDLINE.
Aims : We aimed to explore Yarrowia lipolytica carbonyl reductases as effective biocatalysts and to develop efficient asym. reduction systems for chiral alc. synthesis. Methods and Results : Yarrowia lipolytica carbonyl reductase genes were obtained via homologous sequence amplification strategy. Two carbonyl reductases, YaCRI and YaCRII, were identified and characterized, and used to catalyze the conversion of 2-hydroxyacetophenone (2-HAP) to optically pure (S)-1-phenyl-1,2-ethanediol. Enzymic assays revealed that YaCRI and YaCRII exhibited specific activities of 6·96 U mg-1 (99·8% e.e.) and 7·85 U mg-1 (99·9% e.e.), resp., and showed moderate heat resistance at 40-50°C and acid tolerance at pH 5·0-6·0. An efficient whole-cell two-phase system was established using reductase-expressing recombinant Escherichia coli. The conversion of 2-HAP (20·0 g l-1) conversion with the solvent of di-Bu phthalate was approx. 70-fold higher than in water. Furthermore, the two recombinant E. coli displayed biocatalyst activity and enantioselectivity towards several different carbonyl compounds, and E. coliBL21 (DE3)/pET-28a-yacrII showed a broad substrate spectrum. Conclusions : A new whole-cell recombinant E. coli-based bioreduction system for enantiopure alc. synthesis with high enantioselectivity at high substrate concentrations was developed. Significance and Impact of the Study : We proposed a promising approach for the efficient preparation of enantiopure chiral alcs.
Journal of Applied Microbiology published new progress about 1310357-40-4. 1310357-40-4 belongs to alcohols-buliding-blocks, auxiliary class Benzenes, name is 7-Chloro-2,3,4,5-tetrahydro-1H-benzo[b]azepin-5-ol, and the molecular formula is C24H12, Recommanded Product: 7-Chloro-2,3,4,5-tetrahydro-1H-benzo[b]azepin-5-ol.
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