Sinnett-Smith, James published the artcileCharacterization of a bombesin receptor on Swiss mouse 3T3 cells by affinity cross-linking, Quality Control of 70539-42-3, the publication is Journal of Cellular Biochemistry (1988), 38(4), 237-49, database is CAplus and MEDLINE.
A cell surface protein in Swiss 3T3 cells of apparent Mr 75,000-85,000 was identified previously by chem. crosslinking as a major component of the receptor for peptides of the bombesin family in these cells. Because bombesin-like peptides may interact with other cell surface mols., it was important to establish the correlation between receptor binding and functions of this complex and further characterize the Mr 75,000-85,000 cross-linked protein. Detailed time courses carried out at different temperatures demonstrated that the Mr 75,000-85,000 affinity-labeled band was the earliest cross-linked complex detected in Swiss 3T3 cells incubated with 125I-labeled gastrin-releasing peptide (125I-GRP). Furthermore, the ability of various nonradioactive bombesin agonists and antagonists to block the formation of the Mr 75,000-85,000 cross-linked complex correlated extremely well (r = 0.994) with the relative capacity of these peptides to inhibit 125I-GRP specific binding. Pretreatment with unlabeled GRP for up to 6 h caused only a slight decrease in both specific 125I-GRP binding and the affinity labeling of the Mr 75,000-85,000 protein. The cross-linked complex is a glycoprotein. First, solubilized affinity labeled Mr 75,000-85,000 complex applied to wheat germ lectin-Sepharose columns was eluted by addition of 0.3M N-acetyl-
Journal of Cellular Biochemistry published new progress about 70539-42-3. 70539-42-3 belongs to alcohols-buliding-blocks, auxiliary class pyrrolidine,Ester,Amide,Inhibitor,Inhibitor, name is Bis(2,5-dioxopyrrolidin-1-yl) O,O’-ethane-1,2-diyl disuccinate, and the molecular formula is C8H14O2, Quality Control of 70539-42-3.
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