Extreme dependence of Chloroflexus aggregans LOV domain thermo- and photostability on the bound flavin species was written by Smolentseva, Anastasia;Goncharov, Ivan M.;Yudenko, Anna;Bogorodskiy, Andrey;Semenov, Oleg;Nazarenko, Vera V.;Borshchevskiy, Valentin;Fonin, Alexander V.;Remeeva, Alina;Jaeger, Karl-Erich;Krauss, Ulrich;Gordeliy, Valentin;Gushchin, Ivan. And the article was included in Photochemical & Photobiological Sciences in 2021.Synthetic Route of C9H18O5S The following contents are mentioned in the article:
Light-oxygen-voltage (LOV) domains are common photosensory modules that found many applications in fluorescence microscopy and optogenetics. Here, we show that the Chloroflexus aggregans LOV domain can bind different flavin species (lumichrome, LC; riboflavin, RF; FMN, FMN; FAD, FAD) during heterologous expression and that its physicochem. properties depend strongly on the nature of the bound flavin. We show that whereas the dissociation constants for different chromophores are similar, the melting temperature of the protein reconstituted with single flavin species varies from ∼ 60°C for LC to ∼ 81°C for FMN, and photobleaching half-times vary almost 100-fold. These observations serve as a caution for future studies of LOV domains in non-native conditions yet raise the possibility of fine-tuning various properties of LOV-based fluorescent probes and optogenetic tools by manipulating the chromophore composition This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1Synthetic Route of C9H18O5S).
(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Alcohols are weak acids. The most acidic simple alcohols (methanol and ethanol) are about as acidic as water, and most other alcohols are somewhat less acidic. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Synthetic Route of C9H18O5S
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts