《Development and pre-clinical evaluation of a synthetic oligosaccharide-protein conjugate vaccine against Neisseria meningitidis serogroup C》 was written by Dalal, Juned; Rana, Rakesh; Harale, Kishore; Hanif, Sarmad; Kumar, Nitin; Singh, Deepti; Chhikara, Manoj Kumar. Quality Control of 2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate And the article was included in Vaccine on August 23 ,2019. The article conveys some information:
Significant improvement has been made in the development of vaccines against Neisseria meningitidis infections since the introduction of polysaccharide-protein conjugate vaccines. Conventional bacterial capsular polysaccharide (PS) based conjugate vaccines require unique and expensive manufacturing facilities, complex production processes and extensive quality testing. Synthetic oligosaccharide (OS) based approach is one of the novel technologies that is being developed to simplify production of conjugate vaccines. OSs can be chem. synthesized to a desired length long enough to represent the antigenic epitopes which often present as a homogenous mixture We prepared OSs corresponding to tetramer and octamer of N. meningitidis serogroup C (MenC) PS by organic synthesis. The MenC tetramer and octamer were further conjugated with tetanus toxoid to produce resp. monovalent conjugates having the desired physico-chem. characteristics. The conjugates were evaluated in a mouse model for immunogenicity and compared with a licensed PS conjugate vaccine. Synthetic conjugates could induce anti-MenC PS IgG as well as serum bactericidal titers at levels comparable to those elicited by the licensed vaccine. The increase in length of synthetic oligomers from tetramer to octamer did not appear to increase immunogenicity. The results establish the pre-clin. proof of concept for a synthetic MenC oligosaccharide conjugate vaccine candidate. In the experimental materials used by the author, we found 2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate(cas: 76931-93-6Quality Control of 2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate)
2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate(cas: 76931-93-6) belongs to pyrrolidine. Pyrrolidine on reaction with ketenedithioacetals gave mono- and dipyrrolidino derivatives. Reaction of parent pyrrolidine with alkyl/aryl isocyanates or isothiocyanates provided 1,3-disubstituted ureas/thioureas.Quality Control of 2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate
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