《No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag》 was written by Sheng, Wei; Geiger, James H.; Borhan, Babak. Formula: C4H12KNaO10 And the article was included in Methods in Enzymology in 2020. The article conveys some information:
Fluorescence cell imaging provides a powerful tool to study biol. processes including regulation, protein-protein interaction, trafficking, development, cellular structure and morphol., to name a few. Complimentary to fluorescent proteins (FPs), the development of multiple site-selective labeling techniques offer choice and flexibility in selection of fluorophores for optimal exptl. design. Near-IR (NIR) labels are highly desired since they enable deeper imaging depths and cleaner optical windows. Photochromic labels are also desirable since they provide the capability to control the fluorescence “”turn-ON”” and in some cases “”turn-OFF”” functionality. In addition, no-wash labeling techniques can greatly simplify exptl. procedures and offer real-time imaging options. Also, compared to most of the regular FPs, these systems are often matured rapidly and do not need mol. oxygen for activation. Here, we present a no-wash photochromic NIR fluorescence live cell imaging approach. This method uses engineered human Cellular Retinol Binding Protein II (hCRBPII) as a genetically encodable tag and a solvatochromic dye FR-1V as the fluorophore. At the heart of this system, a photo-triggered switching between NIR “”OFF”” and “”ON”” modes provide spatiotemporal control for subcellular fluorescence imaging. The experimental process involved the reaction of Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5Formula: C4H12KNaO10)
Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5) is a ferroelectric crystal with a high piezoelectric effect and electromechanical coupling coefficient. Formula: C4H12KNaO10 It may be used as a constituent to prepare DNS (3,5- dinitrosalicylic acid) reagent and Fehling′s solution B, which are used in the determination of reducing sugar.
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