On May 31, 2021, Vlckova, Hana Kocova; Catapano, Maria Carmen; Mitasik, Lucia; Kotland, Ondrej; Nejmanova, Iveta; Pourova, Jana; Mladenka, Premysl; Novakova, Lucie published an article.HPLC of Formula: 621-37-4 The title of the article was Featuring ultimate sensitivity of high-resolution LC-MS analysis of phenolics in rat plasma. And the article contained the following:
Sensitive anal. of very low-mol. weight metabolites using liquid chromatog. with quadrupole-time-of-flight mass spectrometry is challenging due to the high losses of ions in a time-of-flight analyzer. Improvement in sensitivity for these analytes via the optimization of advanced parameters, including quadrupole profile, ion guide parameters, and duty cycle, has been achieved. The optimization of the method was carried out using a large spectrum of structurally different compounds including (iso)flavonoids and their known metabolites. These compounds can be categorized into two major groups, i.e., compounds with (iso)flavonoid core and low-mol. weight phenolics. The optimization of the duty cycle enabled up to a 15-fold increase in analyte responses while the contribution of tuning ion optics and quadrupole profile was negligible. The limits of quantifications of our new method were assessed using both standard solutions and rat plasma. They were decreased at least 10 times for several low-mol. weight phenolics enabling measurement of their concentrations in a range of 1-50 ng/mL in rat plasma after protein precipitation Concurrently, the limits of quantifications for compounds with (iso)flavonoid core did not increase distinctly allowing their detection in a range of 0.5-10 ng/mL. The new method was used for the targeting of phenolics in biol. samples from pharmacokinetics experiments The experimental process involved the reaction of 3-Hydroxyphenylacetic acid(cas: 621-37-4).HPLC of Formula: 621-37-4
The Article related to plasma isoflavonoid phenolics lc ms pharmacokinetics, high-resolution mass spectrometry, low-molecular weight analytes, metabolites, phenolics, quadrupole-time-of-flight, Pharmacology: Methods and other aspects.HPLC of Formula: 621-37-4
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