Das, Joydip published the artcile< Identification of alcohol-binding site(s) in proteins using diazirine-based photoaffinity labeling and mass spectrometry>, HPLC of Formula: 25055-82-7, the main research area is protein alc binding site diazirine photoaffinity labeling mass spectrometry; alcoholism; azialcohol; diazirine; mass spectrometry; photoaffinity labeling.
Defining mol. targets of alc. and understanding the mol. mechanism of alc. actions are necessary to develop effective therapeutics for alc. use disorder (AUD). Here, we describe a detailed protocol for identifying alc.-binding site(s) in proteins using diazirine-based azialc. as photoaffinity labeling agents. Upon photoirradiation, azialc. photoincorporates into alc.-binding proteins. The stoichiometry and site of azialc. photoincorporation can be determined using high-resolution mass spectrometry. Identification of the alc.-binding residues in protein followed by measuring the biol. significance of these residues in regulating alc. action are important steps in characterizing the mol. targets of alc.
Chemical Biology & Drug Design published new progress about Algorithm (SEQUEST). 25055-82-7 belongs to class alcohols-buliding-blocks, and the molecular formula is C4H8N2O, HPLC of Formula: 25055-82-7.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts