Gupta, Sampa; Maurya, Pooja; Upadhyay, Akanksha; Kushwaha, Pragati; Krishna, Shagun; Siddiqi, Mohammad Imran; Sashidhara, Koneni V.; Banerjee, Dibyendu published an article in European Journal of Medicinal Chemistry. The title of the article was 《Synthesis and bio-evaluation of indole-chalcone based benzopyrans as promising antiligase and antiproliferative agents》.Reference of 5-(tert-Butyl)-4-hydroxyisophthalaldehyde The author mentioned the following in the article:
DNA replication and repair are complex processes accomplished by the concerted action of a network of enzymes and proteins. DNA ligases play a crucial role in these processes by catalyzing the nick joining between DNA strands. As compared to normal cells, elevated levels of human DNA ligase I (hLigI) is reported in some cancers. The authors studied the inhibition of hLigI mediated DNA nick sealing activity followed by the antiproliferative activity of novel indole-chalcone based benzopyran compounds on cancer cells. One mol. called compound 27 ((E)-2-amino-8-(tert-butyl)-4-(2-methyl-1H-indol-3-yl)-6-(3-oxo-3-(thien-2-yl)prop-1-en-1-yl)-4H-chromene-3-carbonitrile) showed a notable preference for inhibition of hLigI as compared to other ligases and showed enhanced cytotoxicity against colon cancer (DLD-1) cells as compared to normal cells. Mechanistic studies showed that compound 27 directly interacts with hLigI in a competitive manner and did not interact with the DNA substrate during ligation. This novel and potent hLigI inhibitor showed significant inhibition of both monolayer culture as well as 3D culture of DLD-1 cells that mimic solid tumor. It also affected the migration of DLD-1 cells indicating the potential anti-metastatic activity. This novel hLigI inhibitor could therefore serve as a promising lead for anticancer drug development. In the part of experimental materials, we found many familiar compounds, such as 5-(tert-Butyl)-4-hydroxyisophthalaldehyde(cas: 153759-59-2Reference of 5-(tert-Butyl)-4-hydroxyisophthalaldehyde)
5-(tert-Butyl)-4-hydroxyisophthalaldehyde(cas: 153759-59-2) belongs to phenols.Reference of 5-(tert-Butyl)-4-hydroxyisophthalaldehydeDeprotonation of a phenol forms a corresponding negative phenolate ion or phenoxide ion, and the corresponding salts are called phenolates or phenoxides (aryloxides according to the IUPAC Gold Book).
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts