Zheng, Qizhen; Pang, Zhengyuan; Liu, Jingwei; Zhou, Yi; Sun, Yang; Yin, Zheng; Lou, Zhiyong published the artcile< Photoaffinity palladium reagents for capture of protein-protein interactions>, HPLC of Formula: 25055-82-7, the main research area is photoaffinity palladium reagent cysteine containing protein interaction.
Protein-protein interactions (PPIs) are indispensable in almost all cellular processes. Probing of complex PPIs provides new insights into the biol. system of interest and paves the way for the development of therapeutics. Herein, the authors report a strategy for the capture of protein-protein interactions using photoaffinity palladium reagents. First, the palladium-mediated reagent site specifically transferred a photoaffinity modified aryl group to the designated cysteine residue. Next, the photoaffinity group was activated by UV radiation to trap the proximal protein residue for the formation of a crosslink. This strategy was used to capture the PYL-ABA-PP2C interaction, which is at the core of the abscisic acid (ABA) signaling pathway. The authors’ results indicated that this palladium-mediated strategy can serve as an alternative for incorporating an increasing number of diverse substrates for protein crosslinking through cysteine modifications and can be explored for use in mapping protein-peptide or protein-protein interaction surfaces and in trapping potential interacting partners.
Organic & Biomolecular Chemistry published new progress about Photocrosslinking. 25055-82-7 belongs to class alcohols-buliding-blocks, and the molecular formula is C4H8N2O, HPLC of Formula: 25055-82-7.
Referemce:
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