Giamaki, Despoina;Dindini, Konstantina;Samanidou, Victoria F.;Touraki, Maria published 《Simultaneous Quantification of Bisphenol-A and 4-Tert-Octylphenol in the Live Aquaculture Feed Artemia franciscana and in Its Culture Medium Using HPLC-DAD》 in 2022. The article was appeared in 《Methods and Protocols》. They have made some progress in their research.COA of Formula: C14H22O The article mentions the following:
Aquaculture, a mass supplier of seafood, relies on plastic materials that may contain the endocrine disruptors bisphenol-A (BPA) and tert-octylphenol (t-OCT). These pollutants present toxicity to Artemia, the live aquaculture feed, and are transferred through it to the larval stages of the cultured organisms. The purpose of this work is the development and validation of an anal. method to determine BPA and t-OCT in Artemia and their culture medium, using n-octylphenol as the internal standard Extraction of the samples was performed with H2O/TFA (0.08%)-methanol (3:1), followed by SPE. Anal. was performed in a Nucleosil column with mobile phases A (95:5, volume/volume, 0.1% TFA in H2O:CH3CN) and B (5:95, volume/volume, 0.08% TFA in H2O:CH3CN). Calibration curves were constructed in the range of concentrations expected following a 24 h administration of BPA (10 μg/mL) or t-OCT (0.5 μg/mL), below their resp. LC50. At the end of exposure to the pollutants, their total levels appeared reduced by about 32% for BPA and 35% for t-OCT, and this reduction could not be accounted for by photodegradation (9-19%). The developed method was validated in terms of linearity, accuracy, and precision, demonstrating the uptake of BPA and t-OCT in Artemia. And 4-tert-Octylphenol (cas: 140-66-9) was used in the research process.
4-tert-Octylphenol(cas: 140-66-9) is a common environmental pollutant showing weak estrogenic effects.COA of Formula: C14H22O It has been shown to cause harm to vertebrate male reproductive systems.
Reference:
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