Method development for the simultaneous estimation of ospemifine by using RP-HPLC was written by Chaitanya, Mitta;Swathi, Kalepu;Raju, P. Narayan;Swathi, Koduru. And the article was included in International Journal of Pharma Research and Health Sciences in 2015.Recommanded Product: (Z)-2-(4-(4-Chloro-1,2-diphenylbut-1-en-1-yl)phenoxy)ethan-1-ol This article mentions the following:
A selective and sensitive stability-indicating high-performance liquid chromatog. method was developed and validated for the determination of Ospemifine. 10 mg of Ospemifine was dissolved in mobile phase. The solution was scanned from 200-400 nm the spectrum was obtained. The overlay spectrum was used for selection of wavelength for Ospemifine. The isobestic point was taken as detection wavelength. The separation was good, peak shape was good, so we conclude that there is no required for decrease the retention times of peak, so it is taken as final method. Mix a mixture of 40 mL water (40%) and 60 mL of Acetonitrile (60%) and degassed in ultrasonic water bath for 5 min. Filter through 0.22 μ filter under vacuum filtration.10 mg of Ospemifine working standard was accurately weighed and transferred into a 10 mL clean dry volumetric flask and add about 2 mL of diluent and sonicate to dissolve it completely and make volume up to the mark with the same solvent (Stock solution). Further pipet out 1.0 mL from the above stock solution into a 10 mL volumetric flask and was diluted up to the mark with diluent. The chromatog. method development for the estimation of Ospemifine were optimized by several trials for various parameters as different column, flow rate and mobile phase, finally the following chromatog. method was selected for the separation and quantification of Ospemifine in API and pharmaceutical dosage form by RP-HPLC method. The retention time of Ospemifine was found to be 2.425 mins. The system suitability parameters for Ospemifine such as theor. plates and tailing factor were found to be 4146, 1.2. The % purity Ospemifine in pharmaceutical dosage form was found to be 99.56%. In the experiment, the researchers used many compounds, for example, (Z)-2-(4-(4-Chloro-1,2-diphenylbut-1-en-1-yl)phenoxy)ethan-1-ol (cas: 128607-22-7Recommanded Product: (Z)-2-(4-(4-Chloro-1,2-diphenylbut-1-en-1-yl)phenoxy)ethan-1-ol).
(Z)-2-(4-(4-Chloro-1,2-diphenylbut-1-en-1-yl)phenoxy)ethan-1-ol (cas: 128607-22-7) belongs to alcohols. Alcohols are weak acids. The most acidic simple alcohols (methanol and ethanol) are about as acidic as water, and most other alcohols are somewhat less acidic. A multistep synthesis may use Grignard-like reactions to form an alcohol with the desired carbon structure, followed by reactions to convert the hydroxyl group of the alcohol to the desired functionality.Recommanded Product: (Z)-2-(4-(4-Chloro-1,2-diphenylbut-1-en-1-yl)phenoxy)ethan-1-ol
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts