Evaluation and Efficacy Modified Carvacrol and Anti-cancer Peptide Against Cell Line Gastric AGS was written by Elahi, Mona Ghods;Hekmati, Malak;Esmaeili, Davoud;Ziarati, Parisa;Yousefi, Mohamad. And the article was included in International Journal of Peptide Research and Therapeutics in 2022.Category: alcohols-buliding-blocks This article mentions the following:
Cancer is a complex of malignant cell growth and is one of the most challenging issues worldwide. This study aimed to design, determine, and synthesize a modified carvacrol-containing anticancer peptide sequence and its anti-cancer properties investigated against gastric cancer cell line by Real-Time RT PCR technique. Anticancer peptide sequence designed by ACAN bioinformatics software. Modified carvacrol structure designed by bioinformatics software and the anti-cancer sequence added to it. After determining the chem. and structural properties and modeling by bioinformatics software the compound was synthesized. Then, an MTT test was performed on the gastric cancer cell line. In the next step, the AGS cancer cell is exposed to the MTT concentration of the compound in a culture medium. Then, mRNA is extracted from treatment-exposed cancer cells and treatment-free cell lines, and cDNA was then synthesized from mRNA. The effect of the compound on caspase3 and bcl2 apoptosis genes is investigated by Real-Time RT PCR. Alkylation of o-cresol with isopropanol over AlCl3 as an efficient catalyst leads to the appropriate preparation of carvacrol with proper yields (75.6). Results showed that carvacrol accompanied by Anticancer peptide at a concentration of 2.5 mg/mL had cytotoxic effects on the cervical. The inhibitory effects of carvacrol and anticancer peptide increased against inhibition of the Bax gene by 256-fold and caspase 3 by 32-fold and bcl2 decreased by eightfold. Because of the anti-apoptotic properties of modified carvacrol-containing anticancer peptide, it can be used to inhibit cancer cells as a therapeutic supplement and prevention. In the experiment, the researchers used many compounds, for example, 5-Isopropyl-2-methylphenol (cas: 499-75-2Category: alcohols-buliding-blocks).
5-Isopropyl-2-methylphenol (cas: 499-75-2) belongs to alcohols. Under appropriate conditions, inorganic acids also react with alcohols to form esters. To form these esters, a wide variety of specialized reagents and conditions can be used. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Category: alcohols-buliding-blocks
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts