Enzyme activation by water-mimicking dual-functionalized ionic liquids was written by Zhao, Hua;Martin, Caden J.;Larm, Nathaniel E.;Baker, Gary A.;Trujillo, Tyler C.. And the article was included in Molecular Catalysis in 2021.COA of Formula: C6H15NO This article mentions the following:
Biocatalytic synthesis represents a green alternative to metal-catalyzed reactions. However, enzymes typically display much lower catalytic activities in nonaqueous solvents than in aqueous media. To mimic the aqueous environment for enzyme activation, this study designed a series of sixteen dual-functionalized ionic liquids (ILs) that contain both glycol ether (hydrogen-bond acceptor) and tert-alc. (hydrogen-bond donor) groups. These “water-like” ILs enabled high transesterification activities for immobilized Candida antarctica lipase B (CALB) known as Novozym 435 and immobilized Bacillus licheniformis protease (known as subtilisin A), resp. Several water-mimicking ILs containing 2-3 vol% water significantly increased the CALB activity by 1.8-fold of that in tert-butanol, and 1.6-fold of that in diisopropyl ether (both organic solvents are highly enzyme-compatible). To a smaller degree, subtilisin was activated by these ionic solvents up to 1.2-fold (with 100% selectivity at 2 vol% water) than by diisopropyl ether. Fluorescence emission spectra suggested that the characteristic emission maximum peaks were maintained in “water-like” ILs in most cases. In the experiment, the researchers used many compounds, for example, 1-(Dimethylamino)-2-methylpropan-2-ol (cas: 14123-48-9COA of Formula: C6H15NO).
1-(Dimethylamino)-2-methylpropan-2-ol (cas: 14123-48-9) belongs to alcohols. Because alcohols are easily synthesized and easily transformed into other compounds, they serve as important intermediates in organic synthesis. A multistep synthesis may use Grignard-like reactions to form an alcohol with the desired carbon structure, followed by reactions to convert the hydroxyl group of the alcohol to the desired functionality.COA of Formula: C6H15NO
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts