Food phytochemicals, epigallocatechin gallate and myricetin, covalently bind to the active site of the coronavirus main protease in vitro was written by Kato, Yoji;Higashiyama, Akari;Takaoka, Emi;Nishikawa, Miyu;Ikushiro, Shinichi. And the article was included in Advances in Redox Research in 2021.Related Products of 10083-24-6 This article mentions the following:
SARS-CoV-2 main protease is a possible target for protection against viral infection. This study examined the inhibitory effect of food phytochems. on the main protease of SARS-CoV-2 by determining a cleaved product after chromatog. separation First, 37 phytochems., including glycosides and metabolites, were screened at 20μM; epigallocatechin gallate, myricetin, theaflavin, herbacetin, piceatannol, myricitrin, and isothiocyanates inhibited the enzyme in varying degrees. The IC50 values were estimated from 0.4 to 33.3μM against the 0.5-μM enzyme. The dose-dependent adduction of epigallocatechin gallate and myricetin was confirmed by quinone staining of protein blotted onto a membrane. The enzyme activity was decreased by increasing the concentration of the two phytochems., accompanied by increasing the resp. adducted mol. estimated by intact mass spectrometry. Reduced glutathione canceled the formation of conjugate and the inhibitory effect of epigallocatechin gallate or myricetin on the enzyme, suggesting that the formation of the quinone moiety in the phytochems. is critical for the inhibition. The covalent binding of epigallocatechin gallate or myricetin to the cysteine residue at the active site was confirmed by analyzing peptides from the chymotrypsin-digested main protease. In the experiment, the researchers used many compounds, for example, (E)-4-(3,5-Dihydroxystyryl)benzene-1,2-diol (cas: 10083-24-6Related Products of 10083-24-6).
(E)-4-(3,5-Dihydroxystyryl)benzene-1,2-diol (cas: 10083-24-6) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. Secondary alcohols are easily oxidized without breaking carbon-carbon bonds only as far as the ketone stage. No further oxidation is seen except under very stringent conditions.Related Products of 10083-24-6
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Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts