Rodrigues de Sousa, Ronaldo published the artcileA practical approach to obtain high yield lipase-mediated synthesis of octyl caprylate with Novozym 435, Application of n-Octanol, the main research area is octyl caprylate esterification Novozym 435.
This study evaluated coupled effects of molar ratio of substrates and enzyme loading in a solvent-free system using a simple math. approach to obtain high conversions on octyl caprylate synthesis with Novozym 435. When molar ratios of caprylic acid to n-octanol (1:1 and 1:3) were evaluated with enzyme loadings of 1% to 4% (wt/wt acid), an interdependence between the masses of reagents and the enzymes was observed, that was expressed as a math. relation. The study of this relation, named as SER, indicated a specific range of reaction conditions that resulted in conversions above 90%. The most suitable condition corresponded to an acid:alc. molar ratio of 1:1.3 and an enzyme loading of 1.5%, resulting in 94.5% of conversion at 65°C in 3 h of reaction. A different reaction system (bottle reactor) was used to evaluate the influence of reagents mixture and heat distribution. The use of a bottle reactor allowed yield improvement that reached 99.3%. At this condition, Novozym 435 was reused, without washing steps, in three subsequent batches keeping high conversion. A possible balance between the shift of chem. equilibrium by stoichiometric excess of reagents and enzymic inhibition effects by substrates can be expressed math. in a convenient way, helping to predict the behavior of synthesis in different conditions. The math. relation proposed, SER, allowed the achievement of 99% of conversion on enzymic synthesis of octyl caprylate.
Biocatalysis and Biotransformation published new progress about Optimization. 111-87-5 belongs to class alcohols-buliding-blocks, name is n-Octanol, and the molecular formula is C8H18O, Application of n-Octanol.
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