Bober, Josef R. published the artcileGalactose to tagatose isomerization at moderate temperatures with high conversion and productivity, Application of (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, the main research area is Lactobacillus sakei arabinose isomerase galactose tagatose.
There are many industrially-relevant enzymes that while active, are severely limited by thermodn., kinetic, or stability issues (isomerases, lyases, transglycosidases). In this work, we study Lactobacillus sakei L-arabinose isomerase (LsLAI) for D-galactose to D-tagatose isomerization-that is limited by all three reaction parameters. The enzyme demonstrates low catalytic efficiency, low thermostability at temperatures > 40°C, and equilibrium conversion < 50%. After exploring several strategies to overcome these limitations, we show that encapsulating LsLAI in gram-pos. Lactobacillus plantarum that is chem. permeabilized enables reactions at high rates, high conversions, and elevated temperatures In a batch process, this system enables ∼ 50% conversion in 4 h starting with 300 mM galactose (an average productivity of 37 mM h-1), and 85% conversion in 48 h. We suggest that such an approach may be invaluable for other enzymic processes that are similarly kinetically-, thermodynamically-, and/or stability-limited. Nature Communications published new progress about Growth, microbial. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Application of (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal.
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