Li, Wenwen published the artcileHonokiol Restores Microglial Phagocytosis by Reversing Metabolic Reprogramming, Category: alcohols-buliding-blocks, the main research area is honokiol restores microglial phagocytosis metabolic reprogramming; Honokiol; metabolic reprogramming; microglial phagocytosis; mitochondria.
Dysfunction of microglia has been increasingly recognized as a causative factor in Alzheimerprimes disease (AD); thus, developing medicines capable of restoring microglial functions is critically important and constitutes a promising therapeutic strategy. Honokiol is a natural neuroprotective compound extracted from Magnolia officinalis, which may play roles in AD therapy. This study aimed to evaluate the role and the underlying mechanisms of honokiol in microglial phagocytosis. MTT and flow cytometry were used to assess the cell viability and apoptosis, resp. Phagocytic capacity, mitochondrial reactive oxygen species production, and membrane potential were evaluated using fluorescence microscopy. Seahorse XF24 extracellular flux analyzer was for cell glycolysis and oxidative phosphorylation detection. Mass spectrometry was applied for metabolites measurement. Quant. real-time polymerase chain reaction and western blotting were performed to detect the mRNA and protein level of PPARgamma and PGC1alpha, resp. Honokiol alleviated Abeta42-induced BV2 neurotoxicity. Honokiol promoted phagocytic efficiency of BV2 cells through reversing a metabolic switch from oxidative phosphorylation to anaerobic glycolysis and enhancing ATP production Meanwhile, honokiol reduced mitochondrial reactive oxygen species production and elevated mitochondrial membrane potential. Moreover, honokiol increased the expression of PPARgamma and PGC1alpha, which might play pos. roles in energy metabolism and microglial phagocytosis. In this study, honokiol was identified as an effect natural product capable of enhancing mitochondrial function thus promoting microglial phagocytic function.
Journal of Alzheimer’s Disease published new progress about Alzheimer disease. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Category: alcohols-buliding-blocks.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts