On December 1, 2021, Yuan, Bo; Zhao, Danyue; Lyu, Weiting; Yin, Zhiya; Kshatriya, Dushyant; Simon, James E.; Bello, Nicholas T.; Wu, Qingli published an article.COA of Formula: C8H8O3 The title of the article was Development and validation of a micro-QuEChERS method with high-throughput enhanced matrix removal followed with UHPLC-QqQ-MS/MS for analysis of raspberry ketone-related phenolic compounds in adipose tissues. And the article contained the following:
Raspberry ketone (RK) is a major flavor compound in red raspberries, and it has been marketed as a popular weight-loss dietary supplement with high potential in accumulating in fatty tissues. However, challenges in extracting and characterizing RK and its associated phenolic compounds in fatty tissues persist due to the complex matrix effect. In this work, we reported a high-throughput sample preparation method for RK and 25 related phenolic compounds in white adipose tissues using an improved micro-scale QuEChERS (quick, efficient, cheap, easy, rugged and safe) approach with enhanced matrix removal (EMR)-lipid cleanup in 96-well plates, followed by UHPLC-QqQ-MS/MS anal. The absolute recovery was 73-105% at the extraction step, and achieved 71-96% at the EMR cleanup step. The EMR cleanup removed around 66% of total lipids in the acetonitrile extract as profiled by UHPLC-QTOF-MS/MS. The innovative introduction of a reversed-phase C18 sorbent into the extract significantly improved the analytes’ recovery during SpeedVac drying. The final accuracy achieved 80-120% for most analytes. Overall, this newly developed and validated method could serve as a powerful tool for analyzing RK and related phenolic compounds in fatty tissues. The experimental process involved the reaction of 3-Hydroxyphenylacetic acid(cas: 621-37-4).COA of Formula: C8H8O3
The Article related to rubus adipose tissue ketone quechers uhplc qqq ms, bioavailability, fatty tissue, lipid removal, raspberry ketone, solid phase extraction, uhplc-qqq-ms/ms, Food and Feed Chemistry: Fruits, Vegetables, Legumes, and Nuts and other aspects.COA of Formula: C8H8O3
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