Nonprotein Based Enrichment Method to Analyze Peptide Cross-Linking in Protein Complexes was written by Yan, Funing;Che, Fa-Yun;Rykunov, Dmitry;Nieves, Edward;Fiser, Andras;Weiss, Louis M.;Hogue Angeletti, Ruth. And the article was included in Analytical Chemistry (Washington, DC, United States) in 2009.Computed Properties of C7H7NO4 This article mentions the following:
Crosslinking anal. of protein complexes and structures by tandem mass spectrometry (MS/MS) has advantages in speed, sensitivity, specificity, and the capability of handling complicated protein assemblies. However, detection and accurate assignment of the cross-linked peptides are often challenging due to their low abundance and complicated fragmentation behavior in collision-induced dissociation (CID). To simplify the MS anal. and improve the signal-to-noise ratio of the cross-linked peptides, the authors developed a novel peptide enrichment strategy that utilizes a cross-linker with a cryptic thiol group and using beads modified with a photocleavable cross-linker. The functional cross-linkers were designed to react with the primary amino groups in proteins. Human serum albumin was used as a model protein to detect intra- and intermol. cross-linkages. Use of this protein-free selective retrieval method eliminates the contamination that can result from avidin-biotin based retrieval systems and simplifies data anal. These features may make the method suitable to investigate protein-protein interactions in biol. samples. In the experiment, the researchers used many compounds, for example, 3-(Hydroxymethyl)-4-nitrophenol (cas: 60463-12-9Computed Properties of C7H7NO4).
3-(Hydroxymethyl)-4-nitrophenol (cas: 60463-12-9) belongs to alcohols. Under appropriate conditions, inorganic acids also react with alcohols to form esters. To form these esters, a wide variety of specialized reagents and conditions can be used. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Computed Properties of C7H7NO4
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