Development of a metal/chelate polyhydroxyethylmethacrylate monolith capillary for selective depletion of immunoglobulin G from human plasma for proteomics was written by Khaparde, Ashish;Vijayalakshmi, M. A.;Tetala, Kishore K. R.. And the article was included in Journal of Chromatography A in 2017.Recommanded Product: Diethyleneglycoldiacrylate This article mentions the following:
The authors report the development of a new poly HEMA (HEMA-co-DEGDA-co-DATD) monolith capillary functionalized with “IDA-Cu(II) complex”. Of the two tested crosslinkers (methylene bisacrylamide (MBAAm) and diethylene glycol diacrylate (DEGDA)), the presence of DEGDA has enhanced the monolith rigidity. Structural assembly of these monoliths are organized with highly interconnected large globule like structures and dominated by a macropore region. Iminodiacetic acid (IDA) immobilization was performed using two chem. approaches (1. aldehyde-secondary amine reaction and 2. epoxy-secondary amine reaction). FTIR anal. confirmed successful IDA immobilization in both cases. For the first time, a reaction of secondary amine ligand with aldehyde functional material was successfully reported. Overall, the Cu(II)-IDA monolith capillary showed good permeability (3.05 × 10 -13 m2), high IgG adsorption capacity and reusability even after 5 consecutive adsorption-desorption cycles. The amount of protein (IgG/HSA) adsorbed on Cu(II)-IDA monolith prepared via the two chemistries is almost similar. Using this affinity monolith capillary, the authors selectively depleted ∼95% of IgG from human plasma (dilution of 1:16). In the experiment, the researchers used many compounds, for example, Diethyleneglycoldiacrylate (cas: 4074-88-8Recommanded Product: Diethyleneglycoldiacrylate).
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