Anti-Markovnikov alkene oxidation by metal-oxo-mediated enzyme catalysis was written by Hammer, Stephan C.;Kubik, Grzegorz;Watkins, Ella;Huang, Shan;Minges, Hannah;Arnold, Frances H.. And the article was included in Science (Washington, DC, United States) in 2017.HPLC of Formula: 94022-96-5 This article mentions the following:
Catalytic anti-Markovnikov oxidation of alkene feedstocks could simplify synthetic routes to many important mols. and solve a long-standing challenge in chem. Here we report the engineering of a cytochrome P 450 enzyme by directed evolution to catalyze metal-oxo-mediated anti-Markovnikov oxidation of styrenes with high efficiency. The enzyme uses dioxygen as the terminal oxidant and achieves selectivity for anti-Markovnikov oxidation over the kinetically favored alkene epoxidation by trapping high-energy intermediates and catalyzing an oxo transfer, including an enantioselective 1,2-hydride migration. The anti-Markovnikov oxygenase can be combined with other catalysts in synthetic metabolic pathways to access a variety of challenging anti-Markovnikov functionalization reactions. In the experiment, the researchers used many compounds, for example, 2-(Trifluoromethyl)phenethyl alcohol (cas: 94022-96-5HPLC of Formula: 94022-96-5).
2-(Trifluoromethyl)phenethyl alcohol (cas: 94022-96-5) belongs to alcohols. A strong base can deprotonate an alcohol to yield an alkoxide ion (R―O−). For example, sodamide (NaNH2), a very strong base, abstracts the hydrogen atom of an alcohol. Tertiary alcohols cannot be oxidized at all without breaking carbon-carbon bonds, whereas primary alcohols can be oxidized to aldehydes or further oxidized to carboxylic acids.HPLC of Formula: 94022-96-5
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts