Characterization of microbial intolerances and ruminal dysbiosis towards different dietary carbohydrate sources using an in vitro model was written by Kheirandish, Parisa;Petri, Renee Maxine;Sener-Aydemir, Arife;Schwartz-Zimmermann, Heidi Elisabeth;Berthiller, Franz;Zebeli, Qendrim;Pacifico, Catia. And the article was included in Journal of Applied Microbiology in 2022.Reference of 2216-51-5 This article mentions the following:
This study aimed to characterize the critical points for determining the development of dysbiosis associated with feed intolerances and ruminal acidosis. A metabologenomics approach was used to characterize dynamic microbial and metabolomics shifts using the rumen simulation technique (RUSITEC) by feeding native cornstarch (ST), chem. modified cornstarch (CMS), or sucrose (SU). SU and CMS elicited the most drastic changes as rapidly as 4 h after feeding. This was accompanied by a swift accumulation of D-lactate, and the decline of benzoic and malonic acid. A consistent increase in Bifidobacterium and Lactobacillus as well as a decrease in fibrolytic bacteria was observed for both CMS and ST after 24 h, indicating intolerances within the fiber degrading populations. However, an increase in Lactobacillus was already evident in SU after 8 h. An inverse relationship between Fibrobacter and Bifidobacterium was observed in ST. In fact, Fibrobacter was pos. correlated with several short-chain fatty acids, while Lactobacillus was pos. correlated with lactic acid, hexoses, hexose-phosphates, pentose phosphate pathway (PENTOSE-P-PWY), and heterolactic fermentation (P122-PWY). The feeding of sucrose and modified starches, followed by native cornstarch, had a strong disruptive effect in the ruminal microbial community. Feed intolerances were shown to develop at different rates based on the availability of glucose for ruminal microorganisms. These results can be used to establish patterns of early dysbiosis (biomarkers) and develop strategies for preventing undesirable shifts in the ruminal microbial ecosystem. In the experiment, the researchers used many compounds, for example, (1R,2S,5R)-2-Isopropyl-5-methylcyclohexanol (cas: 2216-51-5Reference of 2216-51-5).
(1R,2S,5R)-2-Isopropyl-5-methylcyclohexanol (cas: 2216-51-5) belongs to alcohols. Alcohols are weak acids. The most acidic simple alcohols (methanol and ethanol) are about as acidic as water, and most other alcohols are somewhat less acidic. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Reference of 2216-51-5
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts