Hu, Huigang published the artcilePolysaccharides from pineapple pomace: new insight into ultrasonic-cellulase synergistic extraction and hypoglycemic activities, SDS of cas: 59-23-4, the main research area is Ananas polysaccharide ultrasonic cellulase extraction hypoglycemic activity; Characterization; Hypoglycemic activity; Pineapple pomace polysaccharides; Response surface methodology; Ultrasonic-cellulase extraction.
This study determined the optimal extraction conditions for ultrasonic-cellulase synergistic extraction of polysaccharides from pineapple pomace (PPP) using Plackett-Burman design and response surface methodol. The optimal hydrolysis temperature, ratio of material to water, pH value, hydrolysis time, ultrasonic power and the additive quantity of cellulase were 50 °C, 1:45 g/mL, 6.0, 100 min, 160 W and 2.0%, resp., giving a extraction yield of 1.10 ± 0.03%. PPP was further isolated and purified by DEAE-52 cellulose and Sephadex G-100 chromatog. columns, revealing four main elution peaks, named PPF0, PPF1, PPP2 and PPF3, were obtained. The mol. weight, monosaccharide compositions, structural features and appearance morphol. of polysaccharide fractions (PPFs) were analyzed by high-performance liquid chromatog. (HPLC), gel permeation chromatog. (GPC), UV spectroscopy, fourier transform IR spectroscopy (FT-IR), and SEM (SEM). Furthermore, the hypoglycemic activities of PPFs with different concentrations were also investigated by insulin resistance HepG2 cells model in vitro. Results showed that PPF0, PPF1, PPF2 and PPF3 were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose with mol. weight of 6.71 × 104, 1.11 × 104, 2.22 × 104 and 5.1 × 103 Da, resp. All of them could alleviate the development of insulin resistance HepG2 cells with a dose-dependent relationship. The glucose consumption increased 46.4%, 50.5%, 82.1% and 53.6%; 86.8%, 81.6%, 86.8% and 84.2% at the concentration of 20 μg/mL, resp., without or with insulin. These results suggested that PPFs can be explored as a potential hypoglycemic agent in biomedical and functional food.
International Journal of Biological Macromolecules published new progress about Ananas comosus. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, SDS of cas: 59-23-4.
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