Wei, Piao-piao published the artcileAnalysis of Metabolites from Direct Fermentation of D-Glucose to 2-Keto-L-Gulonic Acid by HPLC, Application of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Guocheng Gongcheng Xuebao (2013), 13(4), 675-680, database is CAplus.
An HPLC-based method for quant. determination of the 4 metabolites of gluconic acid (GlcA), 2-keto-D-gluconic acid (2-KDG), 2,5-diketo-D-gluconic acid (2,5-DKG) and 2-keto-L-gulonic acid (2-KLG) from direct fermentation of D-glucose to 2-KLG by using HPLC was established. The optimum separation of the 4 substances could be achieved under the conditions of using Aminex HPX-87H column (size 300 mm x7.8 mm) with the temperature of 30 °C, sulfuric acid (50 mmol/L) as mobile phase at the flow rate of 0.2 mL/min, RID-10A detector, and malonic acid as internal standard The anal. results show that the method has average anal. deviation of 1.53%, 0.89% and 1.84% on 2-KDG 2,5-DKG and 2-KLG, resp.
Guocheng Gongcheng Xuebao published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C20H19NO4, Application of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.
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