Zhang, Qian published the artcileSiderophores of Bacillus pumilus promote 2-keto-L-gulonic acid production in a vitamin C microbial fermentation system, Synthetic Route of 526-98-7, the publication is Journal of Basic Microbiology (2022), 62(7), 833-842, database is CAplus and MEDLINE.
The precursor of vitamin C, 2-keto-L-gulonic acid (2-KLG), is bio-converted from L-sorbose by a microbial consortium of Ketogulonicigenium vulgare and a helper strain (Bacillus spp.). Most helper strains produce siderophores. To understand the effects of siderophores on promoting 2-KLG yield, the siderophores of Bacillus pumilus SY-A9 were purified and added to a monoculture fermentation system of K. vulgare 25B-1. The results revealed that the titer of 2-KLG reached 7.18 g/L within 60 h and increased by 71.45% when the added concentration of siderophores was 500μg/L. Moreover, the increased production of 2-KLG was accompanied by the overexpressed iron uptake system-related genes, electron transfer chain-related genes, ATP synthesis enzyme-related genes, antioxidant enzyme-related genes, and 2-KLG producing enzyme-related genes, which reduced oxidative stress and ensured the normal energy metabolism of K. vulgare 25B-1. This study demonstrated that siderophores of the helper strain play a key role in the enhancement of 2-KLG biosynthesis.
Journal of Basic Microbiology published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C8H4FNO3, Synthetic Route of 526-98-7.
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