Dixit, Rakhi Bajpai published the artcileSecretomics: a biochemical footprinting tool for developing microalgal cultivation strategies, Name: (S)-2-hydroxysuccinic acid, the main research area is biochem footprinting microalgal cultivation; Biochemical tool; Bioprocess monitoring; Microalgal cultivation; Operation cost; Principal component biplot.
Microalgae offer a promising source of biofuel and a wide array of high-value biomols. Large-scale cultivation of microalgae at low d. poses a significant challenge in terms of water management. High-d. microalgae cultivation, however, can be challenging due to biochem. changes associated with growth dynamics. Therefore, there is a need for a biomarker that can predict the optimum d. for high biomass cultivation. A locally isolated microalga Cyanobacterium aponinum CCC734 was grown with optimized nitrogen and phosphorus in the ratio of 12:1 for sustained high biomass productivity. To understand d.-associated bottlenecks secretome dynamics were monitored at biomass densities from 0.6 ± 0.1 to 7 ± 0.1 g/L (2 to 22 OD) in batch mode. Liquid chromatog. coupled with mass spectrometry identified 880 exometabolites in the supernatant of C. aponinum CCC734. The PCA anal. showed similarity between exometabolite profiles at low (4 and 8 OD) and mid (12 and 16 OD), whereas distinctly sep. at high biomass concentrations (20 and 22 OD). Ten exometabolites were selected based on their role in influencing growth and are specifically present at low, mid, and high biomass concentrations Taking cues from secretome dynamics, 5.0 ± 0.5 g/L biomass concentration (16 OD) was optimal for C. aponinum CCC734 cultivation. Further validation was performed with a semi-turbidostat mode of cultivation for 29 days with a volumetric productivity of 1.0 ± 0.2 g/L/day. The secretomes-based footprinting tool is the first comprehensive growth study of exometabolite at the mol. level at variable biomass densities. This tool may be utilized in analyzing and directing microalgal cultivation strategies and reduction in overall operating costs.
World Journal of Microbiology & Biotechnology published new progress about Biomass. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Name: (S)-2-hydroxysuccinic acid.
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