Tardo-Dino, Pierre-Emmanuel published the artcileThe effect of a physiological increase in temperature on mitochondrial fatty acid oxidation in rat myofibers, Formula: C4H6O5, the main research area is carnitine malate GTP myofiber temperature mitochondria fatty acid oxidation; fatty acid; heat; mitochondrial uncoupling; oxidative stress; skeletal muscle.
We investigated the effect of temperature increase on mitochondrial fatty acid (FA) and carbohydrate oxidation in the slow-oxidative skeletal muscles (soleus) of rats. We measured mitochondrial respiration at 35°C and 40°C with the physiol. substrates pyruvate + 4 mM malate (Pyr) and palmitoyl-CoA (PCoA) + 0.5 mM malate + 2 mM carnitine in permeabilized myofibers under nonphosphorylating (V0) or phosphorylating (Vmax) conditions. Mitochondrial efficiency was calculated by the respiratory control ratio (RCR = Vmax/V0). We used guanosine triphosphate (GTP), an inhibitor of uncoupling protein (UCP), to study the mechanisms responsible for alterations of mitochondrial efficiency. We measured hydrogen peroxide (H2O2) production under nonphosphorylating and phosphorylating conditions at both temperatures and substrates. We studied citrate synthase (CS) and 3-hydroxyl acyl CoA dehydrogenase (3-HAD) activities at both temperatures Elevating the temperature from 35°C to 40°C increased PCoA-V0 and decreased PCoA-RCR, corresponding to the uncoupling of oxidative phosphorylation (OXPHOS). GTP blocked the heat-induced increase of PCoA-V0. Rising temperature moved toward a Pyr-V0 increase, without significance. Heat did not alter H2O2 production, resulting from either PCoA or Pyr oxidation Heat induced an increase in 3-HAD but not in CS activities. In conclusion, heat induced OXPHOS uncoupling for PCoA oxidation, which was at least partially mediated by UCP and independent of oxidative stress. The classically described heat-induced glucose shift may actually be mostly due to a less efficient FA oxidation These findings raise questions concerning the consequences of heat-induced alterations in mitochondrial efficiency of FA metabolism on thermoregulation.
Journal of Applied Physiology published new progress about Mitochondria. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Formula: C4H6O5.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts