Manhas, Neeraj published the artcileComputationally modeling mammalian succinate dehydrogenase kinetics identifies the origins and primary determinants of ROS production, Recommanded Product: (S)-2-hydroxysuccinic acid, the main research area is succinate dehydrogenase reactive oxygen species mitochondria antioxidant simulation; computational biology; computer modeling; enzyme kinetics; enzyme mechanism; free radicals; hydrogen peroxide; mechanistic regulation; oxidative stress; redox regulation; succinate dehydrogenase (SDH); superoxide; superoxide ion; ubiquinone.
Succinate dehydrogenase (SDH) is an inner mitochondrial membrane protein complex that links the Krebs cycle to the electron transport system. It can produce significant amounts of superoxide (O2-) and hydrogen peroxide (H2O2); however, the precise mechanisms are unknown. This fact hinders the development of next-generation antioxidant therapies targeting mitochondria. To help address this problem, we developed a computational model to analyze and identify the kinetic mechanism of O-2 and H2O2 production by SDH. Our model includes the major redox centers in the complex, namely FAD, three iron-sulfur clusters, and a transiently bound semiquinone. Oxidation state transitions involve a one- or two-electron redox reaction, each being thermodynamically constrained. Model parameters were simultaneously fit to many data sets using a variety of succinate oxidation and free radical production data. In the absence of respiratory chain inhibitors, model anal. revealed the 3Fe-4S iron-sulfur cluster as the primary O-2 source. However, when the quinone reductase site is inhibited or the quinone pool is highly reduced, O-2 is generated primarily by the FAD. In addition, H2O2 production is only significant when the enzyme is fully reduced, and fumarate is absent. Our simulations also reveal that the redox state of the quinone pool is the primary determinant of free radical production by SDH. In this study, we showed the importance of analyzing enzyme kinetics and associated side reactions in a consistent, quant., and biophys. detailed manner using a diverse set of exptl. data to interpret and explain exptl. observations from a unified perspective.
Current Medical Research and Opinion published new progress about Antioxidants. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Recommanded Product: (S)-2-hydroxysuccinic acid.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts