Hao, Pengchao published the artcileAuxin-transporting ABC transporters are defined by a conserved D/E-P motif regulated by a prolylisomerase, Computed Properties of 97-67-6, the main research area is Arabidopsis ABCB1 mutation auxin proline malate prolylisomerase protoplast; ABC transporter; ABCB; FKBP; PPIase; Twisted Dwarf1; auxin; auxin transport; membrane transport; plant biochemistry; post-transcriptional regulation.
The plant hormone auxin must be transported throughout plants in a cell-to-cell manner to affect its various physiol. functions. ABCB transporters are critical for this polar auxin distribution, but the regulatory mechanisms controlling their function is not fully understood. The auxin transport activity of ABCB1 was suggested to be regulated by a phys. interaction with FKBP42/Twisted Dwarf1 (TWD1), a peptidylprolyl cistrans isomerase (PPIase), but all attempts to demonstrate such a PPIase activity byTWD1 have failed so far. By using a structurebased approach, we identified several surface-exposed proline residues in the nucleotide binding domain and linker of Arabidopsis ABCB1, mutations of which do not alter ABCB1 protein stability or location but do affect its transport activity. P1008 is part of a conserved signature D/E-P motif that seems to be specific for auxin-transporting ABCBs, which we now refer to as ATAs. Mutation of the acidic residue also abolishes auxin transport activity by ABCB1. All higher plant ABCBs for which auxin transport has been conclusively proven carry this conserved motif, underlining its predictive potential. Introduction of this D/E-P motif into malate importer, ABCB14, increases both its malate and its background auxin transport activity, suggesting that this motif has an impact on transport capacity. The D/EP1008 motif is also important for ABCB1-TWD1 interactions and activation of ABCB1-mediated auxin transport by TWD1. In summary, our data imply a new function for TWD1 acting as a putative activator of ABCB-mediated auxin transport by cistrans isomerization of peptidyl-prolyl bonds.
Journal of Biological Chemistry published new progress about Arabidopsis. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Computed Properties of 97-67-6.
Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts