Wang, Zixuan published the artcileRatiometric mass spectrometry imaging for stain-free delineation of ischemic tissue and spatial profiling of ischemia-related molecular signatures, Recommanded Product: (S)-2-hydroxysuccinic acid, the main research area is ischemia metabolic dysfunction metabolomics ratiometric mass spectrometry imaging; TCA cycle; ischemia; mass spectrometry imaging; metabolic enzyme; ratiometric analysis.
Mass spectrometry imaging (MSI) serves as an emerging tool for spatial profiling of metabolic dysfunction in ischemic tissue. Prior to MSI data anal., commonly used staining methods, e.g., triphenyltetrazole chloride (TTC) staining, need to be implemented on the adjacent tissue for delineating lesion area and evaluating infarction, resulting in extra consumption of the tissue sample as well as morphol. mismatch. Here, we propose an in situ ratiometric MSI method for simultaneous demarcation of lesion border and spatial annotation of metabolic and enzymic signatures in ischemic tissue on identical tissue sections. In this method, the ion abundance ratio of a reactant pair in the TCA cycle, e.g., fumarate to malate, is extracted pixel-by-pixel from an ambient MSI dataset of ischemic tissue and used as a surrogate indicator for metabolic activity of mitochondria to delineate lesion area as if the tissue has been chem. stained. This method is shown to be precise and robust in identifying lesions in brain tissues and tissue samples from different ischemic models including heart, liver, and kidney. Furthermore, the proposed method allows screening and predicting metabolic and enzymic alterations which are related to mitochondrial dysfunction. Being capable of concurrent lesion identification, in situ metabolomics anal., and screening of enzymic alterations, the ratiometric MSI method bears great potential to explore ischemic damages at both metabolic and enzymic levels in biol. research.
Frontiers in Chemistry (Lausanne, Switzerland) published new progress about Amino acids Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Recommanded Product: (S)-2-hydroxysuccinic acid.
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