Beale, David J.; Shah, Rohan; Karpe, Avinash V.; Hillyer, Katie E.; McAuley, Alexander J.; Au, Gough G.; Marsh, Glenn A.; Vasan, Seshadri S. published an article in 2021, the title of the article was Metabolic profiling from an asymptomatic ferret model of SARS-CoV-2 infection.HPLC of Formula: 621-37-4 And the article contains the following content:
Coronavirus disease (COVID-19) is a contagious respiratory disease that is causing significant global morbidity and mortality. Understanding the impact of the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection on the host metabolism is still in its infancy but of great importance. Herein, we investigated the metabolic response during viral shedding and post-shedding in an asymptomatic SARS-CoV-2 ferret model (n = 6) challenged with two SARS-CoV-2 isolates. Virol. and metabolic analyses were performed on (minimally invasive) collected oral swabs, rectal swabs, and nasal washes. Fragments of SARS-CoV-2 RNA were only found in the nasal wash samples in four of the six ferrets, and in the samples collected 3 to 9 days post-infection (referred to as viral shedding). Central carbon metabolism metabolites were analyzed during viral shedding and post-shedding periods using a dynamic Multiple Reaction Monitoring (dMRM) database and method. Subsequent untargeted metabolomics and lipidomics of the same samples were performed using a Liquid Chromatog. Quadrupole Time-of-Flight Mass Spectrometry (LC-QToF-MS) methodol., building upon the identified differentiated central carbon metabolism metabolites. Multivariate anal. of the acquired data identified 29 significant metabolites and three lipids that were subjected to pathway enrichment and impact anal. The presence of viral shedding coincided with the challenge dose administered and significant changes in the citric acid cycle, purine metabolism, and pentose phosphate pathways, amongst others, in the host nasal wash samples. An elevated immune response in the host was also observed between the two isolates studied. These results support other metabolomic-based findings in clin. observational studies and indicate the utility of metabolomics applied to ferrets for further COVID-19 research that advances early diagnosis of asymptomatic and mild clin. COVID-19 infections, in addition to assessing the effectiveness of new or repurposed drug therapies. The experimental process involved the reaction of 3-Hydroxyphenylacetic acid(cas: 621-37-4).HPLC of Formula: 621-37-4
The Article related to covid 19 asymptomatic metabolomics viral shedding, covid-19, sars-cov-2, animal model, ferret, lipidomics, metabolomics, systems biology, Placeholder for records without volume info and other aspects.HPLC of Formula: 621-37-4
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