On July 15, 2020, Yuan, Bo; Zhao, Danyue; Kshatriya, Dushyant; Bello, Nicholas T.; Simon, James E.; Wu, Qingli published an article.Name: 3-Hydroxyphenylacetic acid The title of the article was UHPLC-QqQ-MS/MS method development and validation with statistical analysis: Determination of raspberry ketone metabolites in mice plasma and brain. And the article contained the following:
Raspberry ketone (RK) (4-(4-hydroxyphenyl)-2-butanone) is the major compound responsible for the characteristic aroma of red raspberries, and has long been used com. as a flavoring agent and recently as a weight loss supplement. A targeted UHPLC-QqQ-MS/MS method was developed and validated for anal. of RK and 25 associated metabolites in mouse plasma and brain. Dispersion and projection anal. and central composite design were used for method optimization. Random effect anal. of variance was applied for validation inference and variation partition. Within this framework, repeatability, a broader sense of precision, was calculated as fraction of accuracy variance, reflecting instrumental imprecision, compound degradation and carry-over effects. Multivariate correlation anal. and principle component anal. were conducted, revealing underlying association among the manifold of method traits. R programming was engaged in streamlined statistical anal. and data visualization. Two particular phenomena, the analytes’ background existence in the enzyme solution used for phase II metabolites deconjugation, and the noted lability of analytes in pure solvent at 4° vs. elevated stability in biomatrices, were found critical to method development and validation. The approach for the method development and validation provided a foundation for experiments that examine RK metabolism and bioavailability. The experimental process involved the reaction of 3-Hydroxyphenylacetic acid(cas: 621-37-4).Name: 3-Hydroxyphenylacetic acid
The Article related to uhplc mass spectrometry statistical analysis raspberry ketone metabolite, design of experiment, metabolomics, multivariate analysis, random effects anova, surface response modelling and other aspects.Name: 3-Hydroxyphenylacetic acid
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