Dudka, Ilona; Thysell, Elin; Lundquist, Kristina; Antti, Henrik; Iglesias-Gato, Diego; Flores-Morales, Amilcar; Bergh, Anders; Wikstroem, Pernilla; Groebner, Gerhard published the artcile< Comprehensive metabolomics analysis of prostate cancer tissue in relation to tumor aggressiveness and TMPRSS2-ERG fusion status>, Category: alcohols-buliding-blocks, the main research area is proteome metabolome TMPRSS2 ERG phosphocholine hypoxanthine prostate cancer progression; 1H HRMAS NMR; Gleason score; Metabolomics; Prostate cancer; TMPRSS2-ERG.
Abstract: Background: Prostate cancer (PC) can display very heterogeneous phenotypes ranging from indolent asymptomatic to aggressive lethal forms. Understanding how these PC subtypes vary in their striving for energy and anabolic mols. is of fundamental importance for developing more effective therapies and diagnostics. Here, we carried out an extensive anal. of prostate tissue samples to reveal metabolic alterations during PC development and disease progression and furthermore between TMPRSS2-ERG rearrangement-pos. and -neg. PC subclasses. Methods: Comprehensive metabolomics anal. of prostate tissue samples was performed by non-destructive high-resolution magic angle spinning NMR (1H HR MAS NMR). Subsequently, samples underwent moderate extraction, leaving tissue morphol. intact for histopathol. characterization. Metabolites in tissue extracts were identified by 1H/31P NMR and liquid chromatog.-mass spectrometry (LC-MS). These metabolomics profiles were analyzed by chemometric tools and the outcome was further validated using proteomic data from a sep. sample cohort. Results: The obtained metabolite patterns significantly differed between PC and benign tissue and between samples with high and low Gleason score (GS). Five key metabolites (phosphocholine, glutamate, hypoxanthine, arginine and α-glucose) were identified, who were sufficient to differentiate between cancer and benign tissue and between high to low GS. In ERG-pos. PC, the anal. revealed several acylcarnitines among the increased metabolites together with decreased levels of proteins involved in β-oxidation; indicating decreased acyl-CoAs oxidation in ERG-pos. tumors. The ERG-pos. group also showed increased levels of metabolites and proteins involved in purine catabolism; a potential sign of increased DNA damage and oxidative stress. Conclusions: Our comprehensive metabolomic anal. strongly indicates that ERG-pos. PC and ERG-neg. PC should be considered as different subtypes of PC; a fact requiring different, sub-type specific treatment strategies for affected patients.
BMC Cancer published new progress about Acylcarnitines Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 492-62-6 belongs to class alcohols-buliding-blocks, and the molecular formula is C6H12O6, Category: alcohols-buliding-blocks.
Referemce:
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Alcohols – Chemistry LibreTexts