Tag: 200-300

Rifampicin Increases Expression of Plant Codon-Optimized Bacillus thuringiensis δ-Endotoxin Genes in Escherichia coli was written by Singh, Vivek Kumar;Nain, Vikrant;Phanindra, Mullapudi Lakshmi Venkata;Gothandapani, Sellamuthu;Chhapekar, Sushil Satish;Sreevathsa, Rohini;Sambasiva Rao, K. R. S.;Kumar, Polumetla Ananda;Kumar, Awanish. And the article was included in Protein Journal in 2022.Product Details of 367-93-1 The following contents are mentioned in the article:

Transgenic crops expressing Cry δ-endotoxins of Bacillus thuringiensis for insect resistance have been commercialized worldwide with increased crop productivity and spectacular socioeconomic gains. To attain the enhanced level of protein expression, the cry genes have to be extensively modified for RNA stability and translation efficiency in the plant systems. However, such modifications in nucleotide sequences make it difficult to express the cry genes in Escherichia coli because of the presence of E. coli rare codons. Induction of gene expression through the T7 promoter/lac operator system results in high levels of transcription but limits the availability of activated tRNA corresponding to rare codons that leads to translation stalling at ribosomes. In the present study, an Iso-Pr ss-D-1-thiogalactopyranoside (IPTG)/rifampicin combination-based approach was adopted to induce transcription of cry genes through T7 promoter/lac operator while simultaneously inhibiting the transcription of host genes through rifampicin. The results show that the IPTG/rifampicin combination leads to high-level expression of four plant codon-optimized cry genes (cry2Aa, cry1F, cry1Ac, and cry1AcF). Northern blot anal. of the cry gene expressing E. coli samples showed that the RNA expression level in the IPTG-induced samples was higher as compared to that in the IPTG/rifampicin-induced samples. Diet overlay insect bioassay of IPTG/rifampicin-induced Cry toxins with Helicoverpa armigera larvae showed bioactivity (measured as LC₅₀) similar to the previous studies. The experiment has proved that recombinant synthetic gene (plant codon-optimized gene) with the combination of Rifampicin which inhibits DNA-dependent bacterial RNA polymerase and reduces the excessive baggage of translational machinery of the bacterial cell triggers the production of synthetic protein. Purification of protein using high pH buffer increases the solubility of the protein. Further, LC₅₀ anal. shows no reduction of protein activity leads to protein stability. Further, purified cry toxin protein can be used for crop protection against pests and a purified form of the synthetic protein can be used for antibody production and perform the immunoassay for the identification of the transgenic plant. The crystallog. structure of synthetic protein could be used for interaction study with another insect to see insecticidal activity. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1Product Details of 367-93-1).

(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Alcohols are weak acids. The most acidic simple alcohols (methanol and ethanol) are about as acidic as water, and most other alcohols are somewhat less acidic. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.Product Details of 367-93-1

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Reference of 2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehydeIn 2021 ,《Covalent organic framework-graphene oxide composite: A superior adsorption material for solid phase microextraction of bisphenol A》 was published in Talanta. The article was written by Gao, Wei; Cheng, Jiawen; Yuan, Xiaoli; Tian, Yong. The article contains the following contents:

Novel materials with high adsorption and desorption efficiencies are significant for studying compounds at ultra-trace level. Herein, covalent organic framework-graphene oxide (COF-GO) composite materials are synthesized, and tested for solid phase microextraction (SPME) of bisphenol A (BPA) at ultra-trace level. With GO modified successively by 3-aminopropyltriethoxysilane, 1,3,5-triformylphloroglucinol (Tp), and different ratios of COF monomers (Tp and benzidine (BD)), the composites of TpBD-GO-n (n = 1-4) are synthesized. By coating the composites on a glass fiber, the extraction performances of the composites for BPA are tested with constant flow desorption ionization mass spectrometry (CFDI-MS). The extraction efficiency of the composite TpBD-GO-2 is 2.2 and 4.7 times higher than those of TpBD and GO, resp. The chromatog. separation becomes a non-essential step for detection of BPA, the anal. time for each sample is reduced to 8 min. The limits of detection and quantification of MS for BPA anal. are improved to be 22.2 and 73.9 ng L-1. The linear range is extended to be 10.0 μg L-1 (R2 = 0.9990), and the relative standard deviations of one fiber (n = 11) and fiber-to-fiber (n = 8) are 4.3% and 5.6% (1 μg L-1), resp. With this method, ultra-trace levels of BPA present in river water and sea water samples can be successfully detected and quantified. The results indicate that the TpBD-GO-n composites possess superior extraction performance, and their various applications could be expected. The experimental process involved the reaction of 2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehyde(cas: 34374-88-4Reference of 2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehyde)

2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehyde(cas: 34374-88-4) is a member of phloroglucinol derivatives. Phloroglucinol derivatives are a major class of secondary metabolites. Phloroglucinol compounds can be classified into monomeric, dimeric, trimeric and higher phloroglucinols, and phlorotannins.Reference of 2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehyde

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Electric Literature of C4H12KNaO10In 2015 ,《Rochelle salt – a structural reinvestigation with improved tools. I. The high-temperature paraelectric phase at 308 K》 was published in IUCrJ. The article was written by Mo, Frode; Mathiesen, Ragnvald H.; Beukes, Jon Are; Vu, Khanh Minh. The article contains the following contents:

The crystal structure of the high-temperature paraelec. phase of Rochelle salt (K+·Na+·C4H4O6 2-·4H2O) at 308 K has been reinvestigated using synchrotron X-ray diffraction with refinement parameters R(int) = 0.0123, final (shift/e.s.d.)max = 0.019, R 1(all) = 0.0371 and wR 2(all) = 0.0608. The application of a new gas-flow sample cell designed to control both temperature and relative humidity permitted collection of data of excellent quality and enabled unrestrained refinement of all parameters, including those of the isotropic hydrogen atoms. A precise description of the structure has ensued. One K atom is disordered between two symmetry-equivalent sites; three O atoms in three of the four water mols. exhibit very strong anisotropy. Refining one O atom as a split atom was successful, yielding small improvements in the bonding parameters of several H atoms. The H atoms of all water mols. behave as single pairs. Their final U values are of moderate magnitude indicating that these atoms do not participate in the anisotropy of the parent O atoms. It is suggested that the three water O atoms are in part statically disordered, while the bonded H atoms are not. Except for the split K atom and the three water O atoms there is no evidence of general disorder in the structure.Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5Electric Literature of C4H12KNaO10) was used in this study.

Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5) is a ferroelectric crystal with a high piezoelectric effect and electromechanical coupling coefficient. Electric Literature of C4H12KNaO10 As a Biuret reagent, it is used to measure the protein concentration. Furthermore, it is used as laxative and is also used in food industry.

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Alcohol – Wikipedia,
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In 2017,Hu, Lei; Lu, Yue; Li, Xiaona; Liang, Jianwen; Huang, Tao; Zhu, Yongchun; Qian, Yitai published 《Optimization of Microporous Carbon Structures for Lithium-Sulfur Battery Applications in Carbonate-Based Electrolyte》.Small published the findings.Computed Properties of C4H12KNaO10 The information in the text is summarized as follows:

Developing appropriate sulfur cathode materials in carbonate-based electrolyte is an important research subject for lithium-sulfur batteries. Although several microporous carbon materials as host for sulfur reveal the effect, methods for producing microporous carbon are neither easy nor well controllable. Moreover, due to the complexity and limitation of microporous carbon in their fabrication process, there has been rare investigation of effect on electrochem. behavior in the carbonate-based electrolyte for lithium-sulfur batteries by tuning different micropore size(0-2 nm) of carbon host. Here, an immediate carbonization process, self-activation strategy, is demonstrated which can produce microporous carbon for a sulfur host from alkali-complexes. Besides, by changing different alkali-ion in the previous complex, the obtained microporous carbon exhibits a major portion of ultramicropore (< 0.7 nm, from 54.9-25.8%) and it is demonstrated that the micropore structure of the host material plays a vital role in confining sulfur mol. When evaluated as cathode materials in a carbonate-based electrolyte for Li-S batteries, such microporous carbon/sulfur composite can provide high reversible capacity, cycling stability, and good rate capability. The experimental part of the paper was very detailed, including the reaction process of Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5Computed Properties of C4H12KNaO10)

Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5) is a ferroelectric crystal with a high piezoelectric effect and electromechanical coupling coefficient. Computed Properties of C4H12KNaO10 It may be used as a constituent to prepare DNS (3,5- dinitrosalicylic acid) reagent and Fehling′s solution B, which are used in the determination of reducing sugar.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Wu, Xiutao; Gong, Lijie; Chen, Chen; Tao, Ye; Zhou, Wuxi; Kong, Lingyi; Luo, Jianguang published an article in 2021. The article was titled 《Semi-synthesis of harringtonolide derivatives and their antiproliferative activity》, and you may find the article in Molecules.Computed Properties of C4H12KNaO10 The information in the text is summarized as follows:

Harringtonolide (HO), a natural product isolated from Cephalotaxus harringtonia, exhibits potent antiproliferative activity. However, little information has been reported on the systematic structure-activity relationship (SAR) of HO derivatives Modifications on tropone, lactone, and allyl positions of HO (1) were carried out to provide 17 derivatives (2-13, 11a-11f). The in vitro antiproliferative activity against four cancer cell lines (HCT-116, A375, A549, and Huh-7) and one normal cell line (L-02) was tested. Amongst these novel derivatives, compound 6 exhibited comparable cell growth inhibitory activity to HO and displayed better selectivity index (SI = 56.5) between Huh-7 and L-02 cells. The SAR results revealed that the tropone and lactone moieties are essential for the cytotoxic activities, which provided useful suggestions for further structural optimization of HO. The experimental part of the paper was very detailed, including the reaction process of Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5Computed Properties of C4H12KNaO10)

Potassium sodium (2R,3R)-2,3-dihydroxysuccinate tetrahydrate(cas: 6381-59-5) is a ferroelectric crystal with a high piezoelectric effect and electromechanical coupling coefficient. Computed Properties of C4H12KNaO10 It may be used as a constituent to prepare DNS (3,5- dinitrosalicylic acid) reagent and Fehling′s solution B, which are used in the determination of reducing sugar.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

《Cationic Covalent-Organic Framework as Efficient Redox Motor for High-Performance Lithium-Sulfur Batteries》 was written by Liu, Xiao-Fei; Chen, Hong; Wang, Rui; Zang, Shuang-Quan; Mak, Thomas C. W.. Category: alcohols-buliding-blocks And the article was included in Small in 2020. The article conveys some information:

The shuttle effect of soluble lithium polysulfides (LiPSs) leads to the rapid decay of sulfur cathode, severely hindering the practical applications of lithium-sulfur (Li-S) batteries. To this point, a covalent-organic framework (COF) with proper cationic sites, which can be utilized as the cathode host of high-performance Li-S batteries, is reported. The chem. sulfur anchoring within micropores effectively suppresses the dissolution of LiPSs into the electrolyte. During the discharge step, the cationic sites can accept electrons from anode and deliver them to polysulfides to facilitate the polysulfides′ disintegration. Meanwhile, the cationic sites can receive electrons from polysulfides and then send them to the anode during the charge process, which promotes the polysulfides oxidation Thus, both experiments and computational modeling show that the cationic COF can effectively inhibit the shuttle effect of LiPSs and improve the batteries′ performances. Compared with elec. neutral COFs, the cationic COF-based batteries show much better cycling stability even at high c.d., for instance, a high specific capacity of 468 mA h g-1 is retained after 300 cycles at a c.d. of 4.0 C. In the experiment, the researchers used many compounds, for example, 2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehyde(cas: 34374-88-4Category: alcohols-buliding-blocks)

2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehyde(cas: 34374-88-4) is a member of phloroglucinol derivatives. Regarding monomeric phloroglucinols, this group encompasses acryl phloroglucinols, phloroglucinol-terpene adducts, phloroglucinol glycosides, halogenated phloroglucinols, prenylated phloroglucinols, and cyclicroup polyketides.Category: alcohols-buliding-blocks

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

《The adsorption of polar cyclic compounds on iron surfaces from hydrocarbon solutions and their lubricating properties》 was written by Nakayama, Keiji; Studt, Peter. HPLC of Formula: 591-70-8 And the article was included in Wear on April 15 ,1987. The article conveys some information:

The adsorption is studied of polar cyclic organic compounds from hydrocarbon solutions on Fe powder without removal of any oxide film potentially present on the Fe surface. β-Naphthol is chemisorbed on the Fe surface. The adsorption isotherms of cyclohexanol, α-naphthylamine and quinoline can be fitted by a Langmuir equation. From the adsorption data the surface areas occupied by 1 mol. of these compounds are obtained. The polar cyclic compounds investigated reduce the friction of steel sliding over steel under boundary lubrication conditions, but straight chain compounds with polar end groups in cetane are better boundary lubricants. The load-carrying capacity of friction contacts lubricated with polar aromatic compounds depends on the geometry of the contact. Lubrication of flat contacts with aromatic compounds in cetane provides a higher load-carrying capacity than lubrication with stearic acid as an additive. This is due to the formation of C or decomposition products rich in C on the sliding surface by the aromatics Aromatic cyclic compounds give low friction at much higher bulk oil temperatures than straight chain compounds; this must be due to chemisorption or decomposition of the aromatics on the surface. Mixtures of β-naphthol and stearic acid or 1-octadecanol in cetane give low friction at up to 160° bulk oil temperature In the experimental materials used by the author, we found Octadecan-9-ol(cas: 591-70-8HPLC of Formula: 591-70-8)

Octadecan-9-ol(cas: 591-70-8) belongs to hydroxy-containing compounds. Hydroxy groups participate in the dehydration reactions that link simple biological molecules into long chains. The creation of a peptide bond to link two amino acids to make a protein removes the −OH from the carboxy group of one amino acid.HPLC of Formula: 591-70-8

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Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

《Nanoparticle based simple electrochemical biosensor platform for profiling of protein-nucleic acid interactions》 was written by Dai, Yifan; Chiu, Liang-Yuan; Sui, Yongkun; Dai, Quanbin; Penumutchu, Srinivasa; Jain, Niyati; Dai, Liming; Zorman, Christian A.; Tolbert, Blanton S.; Sankaran, R. Mohan; Liu, Chung Chiun. Synthetic Route of C8H9NO5S And the article was included in Talanta on April 1 ,2019. The article conveys some information:

The anal. of protein-nucleic acid interactions is essential for biophysics related research. However, simple, rapid, and accurate methods for quant. anal. of biomol. interactions are lacking. The authors herein establish an electrochem. biosensor approach for protein-nucleic acid binding anal. Nanoparticle based sensors are fabricated by highly-controlled inkjet printing followed by plasma conversion. A novel bioconjugation method is demonstrated as a simple and rapid approach for protein-based biosensor fabrication. As a proof of concept, the authors analyzed the binding interaction between unwinding protein 1 (UP1) and SL3ESS3 RNA, confirming the accuracy of this nanoparticle based electrochem. biosensor approach with traditional biophys. methods. The authors further accurately profiled and differentiated a unique binding interaction pattern of multiple G-tract nucleic acid sequences with heterogeneous nuclear ribonucleoprotein H1. The authors’ study provides insights into a potentially universal platform for in vitro biomol. interaction anal. using a nanoparticle based electrochem. biosensor approach. In addition to this study using 2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate, there are many other studies that have used 2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate(cas: 76931-93-6Synthetic Route of C8H9NO5S) was used in this study.

2,5-Dioxopyrrolidin-1-yl 2-(acetylthio)acetate(cas: 76931-93-6) belongs to pyrrolidine. Pyrrolidine on reaction with ketenedithioacetals gave mono- and dipyrrolidino derivatives. Reaction of parent pyrrolidine with alkyl/aryl isocyanates or isothiocyanates provided 1,3-disubstituted ureas/thioureas.Synthetic Route of C8H9NO5S

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Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Wang, Lvting; Zhang, Lili; Lin, Bizhou; Zheng, Yanzhen; Chen, Jingling; Zheng, Yun; Gao, Bifen; Long, Jinlin; Chen, Yilin published an article in 2021. The article was titled 《Activation of Carbonyl Oxygen Sites in β-Ketoenamine-Linked Covalent Organic Frameworks via Cyano Conjugation for Efficient Photocatalytic Hydrogen Evolution》, and you may find the article in Small.Recommanded Product: 34374-88-4 The information in the text is summarized as follows:

2D covalent organic frameworks (COFs) are drawing intense attention in heterogenous photocatalysis due to their porous, crystalline, and tailor-made structures. For highly efficient solar-to-chem. energy conversion, revealing and modulating active centers in the skeletons of COFs are of great importance but encounter severe challenges. Herein, it is demonstrated that cyano conjugation on a typical β-ketoenamine-linked COF via aldehyde-imine Schiff-base condensation contributes to an enhanced stable photocatalytic H2-evolution rate of 1.8 mmol h-1 g-1 (λ > 420 nm) with a superior apparent quantum yield of 2.12% at 420 nm, compared to pristine COFs. Both exptl. results and d. functional theory calculations disclose that the cyano conjugation can efficiently improve photoinduced charge separation and effectively decrease the energy barrier for H-intermediate generation on the carbonyl oxygen sites of the functionalized COFs. These findings present a precise organic functionalization strategy to optimize active centers on COF-based photocatalysts for the practical applications.2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehyde(cas: 34374-88-4Recommanded Product: 34374-88-4) was used in this study.

2,4,6-Trihydroxybenzene-1,3,5-tricarbaldehyde(cas: 34374-88-4) is a member of phloroglucinol derivatives. Regarding monomeric phloroglucinols, this group encompasses acryl phloroglucinols, phloroglucinol-terpene adducts, phloroglucinol glycosides, halogenated phloroglucinols, prenylated phloroglucinols, and cyclicroup polyketides.Recommanded Product: 34374-88-4

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Alcohol – Wikipedia,
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The author of 《Efficient approach to carbinol derivatives through palladium-catalyzed base-free addition of aryltriolborates to aldehydes》 were Hu, Kun; Ye, Pengqing; Zhen, Qianqian; Yao, Xinrong; Xu, Tong; Shao, Yinlin; Chen, Jiuxi. And the article was published in Molecules in 2017. Reference of (3-Chlorophenyl)(phenyl)methanol The author mentioned the following in the article:

Palladium-catalyzed base-free addition of aryltriolborates to aldehydes was developed, led to a wide range of carbinol derivatives in good to excellent yields. The efficiency of this transformation was demonstrated by compatibility with a wide range of functional groups. The present synthetic route to carbinol derivatives was readily scaled up to gram quantity without difficulty. Thus, this method represented a simple and practical procedure to access carbinol derivatives The experimental process involved the reaction of (3-Chlorophenyl)(phenyl)methanol(cas: 63012-03-3Reference of (3-Chlorophenyl)(phenyl)methanol)

(3-Chlorophenyl)(phenyl)methanol(cas: 63012-03-3) belongs to hydroxy-containing compounds. Hydroxy groups participate in the dehydration reactions that link simple biological molecules into long chains. The joining of a fatty acid to glycerol to form a triacylglycerol removes the −OH from the carboxy end of the fatty acid.Reference of (3-Chlorophenyl)(phenyl)methanol

Referemce:
Alcohol – Wikipedia,
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