Tag: 200-300

An adaptable platform for in-house hepatitis C serology was written by Pedersen, Jannie;Moukandja, Irene Pegha;Ndidi, Stella;Soerensen, Anna-Louise;Koumakpayi, Ismael Herve;Lekana-Douki, Jean-Bernard;Vachon, Marie-Louise;Weis, Nina;Kobinger, Gary;Fausther-Bovendo, Hugues. And the article was included in Journal of Virological Methods in 2022.SDS of cas: 367-93-1 The following contents are mentioned in the article:

Serol.-based diagnosis remains one of the major tools for diagnosis and surveillance of infectious diseases. However, for many neglected diseases no or only few com. assays are available and often with prices prohibiting large scale testing in low and middle-income countries (LMICs). We developed an adaptable enzyme-linked immunoassay (ELISA) using hepatitis C virus (HCV) as a proof-of-concept application. By combining the maltose-binding-protein with a multiepitope HCV protein, we were able to obtain a high concentration of protein suitable for downstream applications. Following optimization, the assay was verified using previously tested human samples from Canada, Denmark and Gabon in parallel with the use of a com. protein. Sensitivity and specificity were calculated to 98% and 97% resp., after accounting for non-specific binding and assay optimization. This study provides a thorough description of the development, and validation of a multiepitope ELISA-based diagnostic assay against HCV, which could be implemented at low cost. The described methodol. can be readily adapted to develop novel ELISA-based diagnostic assays for other infectious pathogens with well-described immunogenic epitopes. This method could improve the diagnosis of neglected diseases for which affordable diagnostic assays are lacking. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1SDS of cas: 367-93-1).

(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Because alcohols are easily synthesized and easily transformed into other compounds, they serve as important intermediates in organic synthesis. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.SDS of cas: 367-93-1

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Alcohol – Wikipedia,
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Improving genomically recoded Escherichia coli to produce proteins containing non-canonical amino acids was written by Perez, Jessica G.;Carlson, Erik D.;Weisser, Oliver;Kofman, Camila;Seki, Kosuke;Des Soye, Benjamin J.;Karim, Ashty S.;Jewett, Michael C.. And the article was included in Biotechnology Journal in 2022.Computed Properties of C9H18O5S The following contents are mentioned in the article:

A genomically recoded Escherichia coli strain that lacks all amber codons and release factor 1 (C321.ΔA) enables efficient genetic encoding of chem. diverse non-canonical amino acids (ncAAs) into proteins. While C321.ΔA has opened new opportunities in chem. and synthetic biol., this strain has not been optimized for protein production, limiting its utility in widespread industrial and academic applications. To address this limitation, the construction of a series of genomically recoded organisms that are optimized for cellular protein production is described. It is demonstrated that the functional deactivation of nucleases (e.g., rne, endA) and proteases (e.g., lon) increases production of wild-type superfolder green fluorescent protein (sfGFP) and sfGFP containing two ncAAs up to ≈5-fold. Addnl., a genomic IPTG-inducible T7 RNA polymerase (T7RNAP) cassette into these strains is introduced. Using an optimized platform, the ability to introduce two identical N₆-(propargyloxycarbonyl)-_L-Lysine residues site specifically into sfGFP with a 17-fold improvement in production relative to the parent strain is demonstrated. The authors envision that their library of organisms will provide the community with multiple options for increased expression of proteins with new and diverse chemistries. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1Computed Properties of C9H18O5S).

(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Under appropriate conditions, inorganic acids also react with alcohols to form esters. To form these esters, a wide variety of specialized reagents and conditions can be used. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.Computed Properties of C9H18O5S

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Alcohol – Wikipedia,
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Preparation of rabbit polyclonal antibody against porcine gasdermin D protein and determination of the expression of gasdermin D in cultured cells and porcine tissues was written by Song, Jiameng;Wang, Kexin;Ma, Bo;Wang, Junwei;Zhang, Wenlong. And the article was included in Protein Expression and Purification in 2021.COA of Formula: C9H18O5S The following contents are mentioned in the article:

Gasdermin-D (GSDMD) is a member of the gasdermin (Gsdm) protein family, and its cleavage by inflammatory cysteine proteases (caspases, CASPs) is a critical event in cell pyroptosis. The role and functions of GSDMD on mice and humans are widely studied, but its expression, structure, and function in other species are less known. In the present work, rabbit anti-porcine GSDMD (pGSDMD) polyclonal antibody was prepared by immunizing New Zealand white rabbits with prokaryotic expressed recombinant pGSDMD (rpGSDMD). The prepared polyclonal antibody showed good specificity in Western blot and indirect immunofluorescence (IIF) assays. Western blot results showed that the polyclonal antibody could recognize overexpressed pGSDMD in human embryonic kidney cells (HEK293T) and endogenously expressed pGSDMD in cultured intestinal porcine enterocytes (IPEC-J2) and porcine kidney cells (PK-15). Western blot also revealed that pGSDMD was expressed in the heart, liver, lung, kidney, gallbladder, and jejunum of pigs. HEK293T cells overexpressing GSDMD showed green fluorescence in the IIF assay only after being treated with 0.3% Triton-X 100, which indicated that the full-length pGSDMD was located in the plasma but not on the cell membrane. This work provides a useful tool and basic information for further studies on pGSDMD. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1COA of Formula: C9H18O5S).

(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Alcohols are among the most common organic compounds. They are used as sweeteners and in making perfumes, are valuable intermediates in the synthesis of other compounds, and are among the most abundantly produced organic chemicals in industry. Under carefully controlled conditions, simple alcohols can undergo intermolecular dehydration to give ethers. This reaction is effective only with methanol, ethanol, and other simple primary alcohols.COA of Formula: C9H18O5S

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Synthesis, spectral characterization and crystal structure of Ni(II) pyridoxal thiosemicarbazone complexes and their recyclable catalytic application in the nitroaldol (Henry) reaction in ionic liquid media was written by Manikandan, Rajendran;Anitha, Panneerselvam;Prakash, Govindan;Vijayan, Paranthaman;Viswanathamurthi, Periasamy. And the article was included in Polyhedron in 2014.Electric Literature of C8H10ClNO3 The following contents are mentioned in the article:

The new square planar nickel(II) complexes, ([NiLPPh₃]: 1, 2 and 3) were synthesized from the reaction of [NiCl₂(PPh₃)₂] with the tridentate Schiff base ligand, pyridoxal thiosemicarbazone (L¹, 4), pyridoxal N-Me thiosemicarbazone (L², 5) and pyridoxal N-Ph thiosemicarbazone (L³, 6) resp. in ethanol. These complexes were characterized by elemental analyses, IR, UV-visible, ¹H NMR, ³¹P NMR and ESI-MS spectroscopy. The mol. structure of the complex [Ni(L²)PPh₃] (2) was determined by single-crystal x-ray diffraction, which reveals a distorted square planar geometry around the nickel(II) ion. The nitroaldol reaction was studied in detail using the nickel(II) complexes as catalysts in a homogeneous solution formed by an ionic liquid and methanol. The effect of solvent, ionic liquid, time, temperature, catalyst loading and substituent of the ligand moiety on the reaction was also studied. The β-nitroalc. products were obtained in good yields of up to 97%. A two step substrate addition mechanism was tentatively proposed based on ESI-MS spectral monitoring of the reaction mass. This study involved multiple reactions and reactants, such as 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5Electric Literature of C8H10ClNO3).

3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5) belongs to alcohols. Similar to water, an alcohol can be pictured as having an sp3 hybridized tetrahedral oxygen atom with nonbonding pairs of electrons occupying two of the four sp3 hybrid orbitals. The most common reactions of alcohols can be classified as oxidation, dehydration, substitution, esterification, and reactions of alkoxides.Electric Literature of C8H10ClNO3

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Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity was written by Heyde, Sophia A. H.;Noerholm, Morten H. H.. And the article was included in Communications Biology in 2021.Safety of (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol The following contents are mentioned in the article:

Gene expression toxicity is an important biol. phenomenon and a major bottleneck in biotechnol. Escherichia coli BL21(DE3) is the most popular choice for recombinant protein production, and various derivatives have been evolved or engineered to facilitate improved yield and tolerance to toxic genes. However, previous efforts to evolve BL21, such as the Walker strains C41 and C43, resulted only in decreased expression strength of the T7 system. This reveals little about the mechanisms at play and constitutes only marginal progress towards a generally higher producing cell factory. Here, we restrict the solution space for BL21(DE3) to evolve tolerance and isolate a mutant strain Evo21(DE3) with a truncation in the essential RNase E. This suggests that RNA stability plays a central role in gene expression toxicity. The evolved rne truncation is similar to a mutation previously engineered into the com. available BL21Star(DE3), which challenges the existing assumption that this strain is unsuitable for expressing toxic proteins. We isolated another dominant mutation in a presumed substrate binding site of RNase E that improves protein production further when provided as an auxiliary plasmid. This makes it easy to improve other BL21 variants and points to RNases as prime targets for cell factory optimization. This study involved multiple reactions and reactants, such as (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1Safety of (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol).

(2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol (cas: 367-93-1) belongs to alcohols. Similar to water, an alcohol can be pictured as having an sp3 hybridized tetrahedral oxygen atom with nonbonding pairs of electrons occupying two of the four sp3 hybrid orbitals. Alcohols may be oxidized to give ketones, aldehydes, and carboxylic acids. These functional groups are useful for further reactions. Oxidation of organic compounds generally increases the number of bonds from carbon to oxygen (or another electronegative element, such as a halogen), and it may decrease the number of bonds to hydrogen.Safety of (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(isopropylthio)tetrahydro-2H-pyran-3,4,5-triol

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Alcohol – Wikipedia,
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Crystal structure, spectroscopic, DNA binding studies and DFT calculations of a Zn(II) complex was written by Mondal, Satyajit;Chakraborty, Moumita;Mondal, Antu;Pakhira, Bholanath;Mukhopadhyay, Subhra Kanti;Banik, Avishek;Sengupta, Swaraj;Chattopadhyay, Shyamal Kumar. And the article was included in New Journal of Chemistry in 2019.Product Details of 65-22-5 The following contents are mentioned in the article:

Herein the authors report, a mononuclear, highly fluorescent zinc(II) complex Zn(L)(N₃)(H₂O) (1) that was prepared by an easy “one pot” method, in which the tridentate Schiff base ligand (E)-4-((2-(dimethylamino)ethylimino)methyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol (HL) was generated in situ. The compound was characterized by various spectroscopic techniques, and its structure was determined by single crystal x-ray diffraction studies. DFT calculations were used to understand the electronic structures of the ligand and the complex, and TD-DFT calculations were performed to interpret the nature of the electronic transitions observed in their UV-visible spectra. In the complex, Zn(II) is pentacoordinated with one azide ligand, an aqua ligand and a monoanionic tridentate N,N,O-donor ligand. In an aqueous methanol (1 : 9 by volume) solution, at the physiol. pH (0.01 M Tris-HCl buffer, pH 7.4), compound 1 exhibits an intense greenish blue fluorescence (λ_ex 390 nm, λ_em 462 nm), whose intensity is ∼17-fold stronger than that of the free ligand. Compound 1 is found to show significant DNA binding activity. The pyridoxal appended tridentate ligand can be used for the bio-imaging of Zn(II). This study involved multiple reactions and reactants, such as 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5Product Details of 65-22-5).

3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5) belongs to alcohols. The oxygen atom of the strongly polarized O―H bond of an alcohol pulls electron density away from the hydrogen atom. This polarized hydrogen, which bears a partial positive charge, can form a hydrogen bond with a pair of nonbonding electrons on another oxygen atom. The most common reactions of alcohols can be classified as oxidation, dehydration, substitution, esterification, and reactions of alkoxides.Product Details of 65-22-5

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Alcohol – Wikipedia,
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Cu(II) complexes of a tridentate N,N,O-donor Schiff base of pyridoxal: synthesis, X-ray structures, DNA-binding properties and catecholase activity was written by Mondal, Satyajit;Chakraborty, Moumita;Mondal, Antu;Pakhira, Bholanath;Blake, Alexander J.;Sinn, Ekkehard;Chattopadhyay, Shyamal Kumar. And the article was included in New Journal of Chemistry in 2018.Related Products of 65-22-5 The following contents are mentioned in the article:

Two new Cu(II) complexes [Cu(L₁)(N₃)] (1) and [Cu(L₁)(NCS)]_n (2), where HL₁ ((E)-4-((2(dimethylamino)ethylimino)methyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol) is an N,N,O-donor Schiff base ligand, were synthesized. These complexes were characterized from their spectroscopic, electrochem., and other physicochem. properties. X-ray crystal structure determination reveals that in complex 1, Cu(II) is in a square planar geometry with the N,N,O-donor ligand and a terminally coordinated azide ion. In complex 2, however, Cu(II) has a square pyramidal geometry, and apart from the tridentate Schiff base ligand there is a thiocyanate ion, which acts in a μ_1,3-bridging mode, connecting the adjacent Cu(II) atoms in an axial-equatorial fashion forming a one dimensional chain. Cyclic voltammetry shows that the complexes undergo Cu(II)/Cu(I) reductions at -0.40 to -0.55 V followed by imine reduction at ∼-0.7 V. Cu(I)/Cu(0) reduction is observed at ∼-0.9 V. Complex 1 also shows appreciable catalytic activity for the aerial oxidation of 3,5 DTBCH₂ to DTBQ (catecholase activity). Both the complexes also show strong binding affinity towards calf-thymus DNA. This study involved multiple reactions and reactants, such as 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5Related Products of 65-22-5).

3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5) belongs to alcohols. Alcohols are among the most common organic compounds. They are used as sweeteners and in making perfumes, are valuable intermediates in the synthesis of other compounds, and are among the most abundantly produced organic chemicals in industry. Grignard and organolithium reagents are powerful tools for organic synthesis, and the most common products of their reactions are alcohols.Related Products of 65-22-5

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Alcohol – Wikipedia,
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Synthesis, structure and in vitro biological activity of pyridoxal N(4)-substituted thiosemicarbazone cobalt(III) complexes was written by Manikandan, Rajendran;Vijayan, Paranthaman;Anitha, Panneerselvam;Prakash, Govindan;Viswanathamurthi, Periasamy;Butcher, Ray Jay;Velmurugan, Krishnaswamy;Nandhakumar, Raju. And the article was included in Inorganica Chimica Acta in 2014.Product Details of 65-22-5 The following contents are mentioned in the article:

Cobalt(III) complexes containing pyridoxal N(4)-substituted thiosemicarbazone ligands with the composition [Co(HL^1-2·Cl)(HL^1-2·H₂O)] (1–2) were synthesized from the reaction of [CoCl₂(PPh₃)₂] and pyridoxal N-methyl-thiosemicarbazone hydrochloride (H₃L¹·Cl)/pyridoxal N-phenyl-thiosemicarbazone hydrochloride (H₃L²·Cl). The richness of the coordination chem. of this ligand is highlighted by the modulation of its charge from neutral (H₃L·Cl) (L) to dianionic (HL·Cl) (L^2-) and monoanionic forms (HL·H₂O) (L^–) and coordinated as tridentate bineg. mode around cobalt(III) ion by forming neutral complex. The new complexes were characterized by various anal. and spectroscopic techniques (IR, electronic, ¹H NMR and ESI-Mass). The x-ray crystal structure of the complex 2, demonstrated distorted octahedral coordination geometry around the metal center. Further, the study of effect of substitution (CH₃ or Ph) on terminal N(4)-nitrogen of thiosemicarbazone exhibited its influence on the potential binding and cleavage ability with DNA, BSA binding, free radical scavenging and cytotoxicity. This study involved multiple reactions and reactants, such as 3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5Product Details of 65-22-5).

3-Hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde hydrochloride (cas: 65-22-5) belongs to alcohols. Alcohols are among the most common organic compounds. They are used as sweeteners and in making perfumes, are valuable intermediates in the synthesis of other compounds, and are among the most abundantly produced organic chemicals in industry. Secondary alcohols are easily oxidized without breaking carbon-carbon bonds only as far as the ketone stage. No further oxidation is seen except under very stringent conditions.Product Details of 65-22-5

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Alcohol – Wikipedia,
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Towards replacement of animal tests with in vitro assays: a gene expression biomarker predicts in vitro and in vivo estrogen receptor activity was written by Corton, J. Christopher;Liu, Jie;Kleinstreuer, Nicole;Gwinn, Maureen R.;Ryan, Natalia. And the article was included in Chemico-Biological Interactions in 2022.SDS of cas: 620-92-8 The following contents are mentioned in the article:

High-throughput transcriptomics (HTTr) has the potential to support efforts to reduce or replace some animal tests. In past studies, we described a computational approach utilizing a gene expression biomarker consisting of 46 genes to predict estrogen receptor (ER) activity after chem. exposure in ER-pos. human breast cancer cells including the MCF-7 cell line. We hypothesized that the biomarker model could identify ER activities of chems. examined by Endocrine Disruptor Screening Program (EDSP) Tier 1 screening assays in which transcript profiles of the same chems. were examined in MCF-7 cells. For the 62 chems. examined including 5 chems. examined in this study using RNA-Seq, the ER biomarker model accuracy was 1) 97% for in vitro reference chems., 2) 76-85% for guideline uterotrophic assays, and 3) 87-88% for guideline and nonguideline uterotrophic assays. For the same chems., these accuracies were similar or slightly better than those of the ToxCast ER model based on 18 in vitro assays. The performance of the ER biomarker model indicates that HTTr interpreted using the ER biomarker correctly identifies active and inactive ER reference chems. As part of the HTTr screening program the approach could rapidly identify chems. with potential ER bioactivities for addnl. screening and testing. This study involved multiple reactions and reactants, such as 4,4′-Methylenediphenol (cas: 620-92-8SDS of cas: 620-92-8).

4,4′-Methylenediphenol (cas: 620-92-8) belongs to alcohols. Alkyl halides are often synthesized from alcohols, in effect substituting a halogen atom for the hydroxyl group. Under carefully controlled conditions, simple alcohols can undergo intermolecular dehydration to give ethers. This reaction is effective only with methanol, ethanol, and other simple primary alcohols.SDS of cas: 620-92-8

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Alcohol – Wikipedia,
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Physiologically Based Toxicokinetic Modeling of Bisphenols in Zebrafish (Danio rerio) Accounting for Variations in Metabolic Rates, Brain Distribution, and Liver Accumulation was written by Chelcea, Ioana;Oern, Stefan;Hamers, Timo;Koekkoek, Jacco;Legradi, Jessica;Vogs, Carolina;Andersson, Patrik L.. And the article was included in Environmental Science & Technology in 2022.Quality Control of 4,4′-Methylenediphenol The following contents are mentioned in the article:

Bisphenol A (BPA) is an industrial chem., which has raised human health and environmental concerns due to its endocrine-disrupting properties. BPA analogs are less well-studied despite their wide use in consumer products. These analogs have been detected in water and aquatic organisms around the world, with some analogs showing toxic effects in various species including fish. Here, we present novel organ-specific time-course distribution data of bisphenol Z (BPZ) in female zebrafish (Danio rerio), including concentrations in the ovaries, liver, and brain, a rarely sampled organ with high toxicol. relevance. Furthermore, fish-specific in vitro biotransformation rates were determined for 11 selected bisphenols. A physiol. based toxicokinetic (PBTK) model was adapted for four of these bisphenols, which was able to predict levels in the gonads, liver, and brain as well as the whole body within a 2-5-fold error with respect to exptl. data, covering several important target organs of toxicity. In particular, predicted liver concentrations improved compared to currently available PBTK models. Predicted data indicate that studied bisphenols mainly distribute to the carcass and gonads and less to the brain. Our model provides a tool to increase our understanding on the distribution and kinetics of a group of emerging pollutants. This study involved multiple reactions and reactants, such as 4,4′-Methylenediphenol (cas: 620-92-8Quality Control of 4,4′-Methylenediphenol).

4,4′-Methylenediphenol (cas: 620-92-8) belongs to alcohols. Because alcohols are easily synthesized and easily transformed into other compounds, they serve as important intermediates in organic synthesis. A multistep synthesis may use Grignard-like reactions to form an alcohol with the desired carbon structure, followed by reactions to convert the hydroxyl group of the alcohol to the desired functionality.Quality Control of 4,4′-Methylenediphenol

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Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts