Category: 526-98-7

Uroz, Stephane published the artcileDual transcriptomics and proteomics analyses of the early stage of interaction between Caballeronia mineralivorans PML1(12) and mineral, Computed Properties of 526-98-7, the publication is Environmental Microbiology (2020), 22(9), 3838-3862, database is CAplus and MEDLINE.

Summary : Minerals and rocks represent essential reservoirs of nutritive elements for the long-lasting functioning of forest ecosystems developed on nutrient-poor soils. While the presence of effective mineral weathering bacteria was evidenced in the rhizosphere of different plants, the mol. mechanisms involved remain uncharacterized. To fill this gap, we combined transcriptomic, proteomics, geo-chem. and physiol. analyses to decipher the potential mol. mechanisms explaining the mineral weathering effectiveness of strain PML1(12) of Caballeronia mineralivorans. Considering the early-stage of the interaction between mineral and bacteria, we identified the genes and proteins differentially expressed when: (i) the environment is depleted of certain essential nutrients (i.e., Mg and Fe), (ii) a mineral is added and (iii) the carbon source (i.e., glucose vs mannitol) differs. The integration of these data demonstrates that strain PML1(12) is capable of (i) mobilizing iron through the production of a non-ribosomal peptide synthetase-independent siderophore, (ii) inducing chemotaxis and motility in response to nutrient availability and (iii) strongly acidifying its environment in the presence of glucose using a suite of GMC oxidoreductases to weather mineral. These results provide new insights into the mol. mechanisms involved in mineral weathering and their regulation and highlight the complex sequence of events triggered by bacteria to weather minerals.

Environmental Microbiology published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C3H5BN2O2, Computed Properties of 526-98-7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Lowenthal, Mark S. published the artcileDeveloping qualitative LC-MS methods for characterization of Vaccinium berry Standard Reference Materials, HPLC of Formula: 526-98-7, the publication is Analytical and Bioanalytical Chemistry (2013), 405(13), 4451-4465, database is CAplus and MEDLINE.

Standard Reference Materials (SRMs) offer the scientific community a stable and homogeneous source of material that holds countless application possibilities. Traditionally, the National Institute of Standards and Technol. (NIST) has provided SRMs with associated quant. information (certified values) for a select group of targeted analytes as measured in a solution or complex matrix. While the current needs of the SRM community are expanding to include non-quant. data, NIST is attempting to broaden the scope of how and what information is offered to the SRM community by providing qual. information about biomaterials, such as chromatog. fingerprints and profiles of untargeted identifications. In this work, metabolomic and proteomic profiling efforts were employed to characterize a suite of six Vaccinium berry SRMs. In the discovery phase, liquid chromatog.-tandem mass spectrometry (LC-MS/MS) data was matched to mass spectral libraries; a subsequent validation phase based on multiple-reaction monitoring LC-MS/MS relied on both retention time matching of authentic standards along with fragmentation data for a qual. overview of the most prominent organic compounds present. Definitive and putative identifications were determined for over 70 metabolites based on reporting guidelines set forth by the Metabolomics Standards Initiative (Metabolomics 3(3):211-221, 2007), and the capability of electrospray ionization mass spectrometry (ESI-MS) to profile untargeted metabolites within a complex matrix using mass spectral matching is demonstrated. Bottom-up proteomic analyses were possible using peptide databases translated from expressed sequence tags (ESTs). Homol. searches provided identification of novel Vaccinium proteins based on homol. to related genera. Chromatog. fingerprints of these berry materials were acquired for supplemental qual. information to be provided to users of these SRMs. An unbounded set of qual. data about a biomaterial is a valuable complement to quant. information traditionally provided in NIST Certificates of Anal.

Analytical and Bioanalytical Chemistry published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, HPLC of Formula: 526-98-7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Feng, Qiangqiang published the artcileKey variables prediction based on mechanism models of 2-KGA mixed culture fermentation, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Huagong Xuebao (Chinese Edition) (2013), 64(8), 2913-2917, database is CAplus.

2-Keto-L-gulonic acid (2-KGA), the precursor for vitamin C synthesis, is produced by the mixed culture of Ketogulonicigenium vulgare and Bacillus megaterium. In this paper, the previously established kinetic model for 2-KGA mixed culture was firstly tested with the data of 80 industrial batches. Based on sensitivity anal., it was found that some insensitive parameters might be assigned fixed values to minimize computing time. Then, the model was used to predict the most important state variables, i.e., substrate and product concentrations Moving data window technique and rolling parameter identification approach were used in the prediction process 4 h and 8 h ahead prediction errors for 2-KGA concentration were less than 5%.

Huagong Xuebao (Chinese Edition) published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Name: (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Feng, Qiangqiang published the artcileModeling on interaction of different strains in 2-KGA mixed culture fermentation, Category: alcohols-buliding-blocks, the publication is Huagong Xuebao (Chinese Edition) (2013), 64(7), 2520-2525, database is CAplus.

The interaction between Bacillus megaterium and Ketogulonicigenium vulgare was studied for 2-keto-L-gulonic acid (2-KGA) mixed culture fermentation Bacillus megaterium might adjust its growth behavior by quorum sensing. Metabolites and autolysis substances of Bacillus megaterium were beneficial to overcoming the metabolic defects of Ketogulonicigenium vulgare and therefore accelerating the latter growth. Ketogulonicigenium vulgare released lysozyme to promote Bacillus megaterium autolysis. Bacillus megaterium autolysis released specific protease substances to enhance sorbitol dehydrogenase (SDH) which might increase the synthesis rate of 2-KGA. Based on such mechanisms, a kinetic model of 2-KGA mixed fermentation was established. Model validation was carried out with four sets of exptl. data under different cultivation conditions. The results demonstrated that the proposed model was able to well describe the growth of two bacteria and 2-KGA production

Huagong Xuebao (Chinese Edition) published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Category: alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Kim, Sung Phil published the artcileComposition of Herba Pogostemonis Water Extract and Protection of Infected Mice against Salmonella Typhimurium-Induced Liver Damage and Mortality by Stimulation of Innate Immune Cells, HPLC of Formula: 526-98-7, the publication is Journal of Agricultural and Food Chemistry (2012), 60(49), 12122-12130, database is CAplus and MEDLINE.

GC-MS anal. of a hot water extract of Herba Pogostemonis (HP) revealed the presence of 131 compounds HP slightly inhibited Salmonella Typhimurium bacteria in culture and stimulated uptake of the bacteria into RAW 264.7 murine macrophage cells as indicated by both increased fluorescence from internalized FITC-dextran and increased colony-forming unit (CFU) counts of the lysed macrophages. Postinfection, the HP-treated cells showed lower bacterial counts than the control. HP elicited altered morphol., elevated inducible NO synthase (iNOS) mRNA, and reduced pro-inflammatory cytokine expression in macrophage cells. Salmonella induced increased expression of iNOS mRNA, cognate polypeptides, and NO. Histol. of mice infected with a sublethal dose (1 × 10⁴ CFU) of Salmonella showed that i.p. administered HP protected against necrosis of the liver, a biomarker of in vivo salmonellosis. The lifespan of mice infected with a LD (1 × 10⁵ CFU) was significantly extended. These results suggest that the activity of HP against bacterial infection in mice occurs through the activation of innate immune macrophage cells. The relationship of composition of HP to bioactivity is discussed.

Journal of Agricultural and Food Chemistry published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, HPLC of Formula: 526-98-7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Wei, Piao-piao published the artcileAnalysis of Metabolites from Direct Fermentation of D-Glucose to 2-Keto-L-Gulonic Acid by HPLC, Application of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Guocheng Gongcheng Xuebao (2013), 13(4), 675-680, database is CAplus.

An HPLC-based method for quant. determination of the 4 metabolites of gluconic acid (GlcA), 2-keto-D-gluconic acid (2-KDG), 2,5-diketo-D-gluconic acid (2,5-DKG) and 2-keto-L-gulonic acid (2-KLG) from direct fermentation of D-glucose to 2-KLG by using HPLC was established. The optimum separation of the 4 substances could be achieved under the conditions of using Aminex HPX-87H column (size 300 mm x7.8 mm) with the temperature of 30 °C, sulfuric acid (50 mmol/L) as mobile phase at the flow rate of 0.2 mL/min, RID-10A detector, and malonic acid as internal standard The anal. results show that the method has average anal. deviation of 1.53%, 0.89% and 1.84% on 2-KDG 2,5-DKG and 2-KLG, resp.

Guocheng Gongcheng Xuebao published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C20H19NO4, Application of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

An, Zhi-min published the artcileStructural changes of 2-keto-L-gulonic acid studied by NMR spectroscopy, Formula: C6H10O7, the publication is Bopuxue Zazhi (2014), 31(3), 407-414, database is CAplus.

2-Keto-L-gulonic acid (2-KLG) changes its mol. structure in many solvents at room temperature A five-membered intramol. ring structure can easily be formed by the 2,5-hemiacetal reaction after the opening of the carbonyl group in 2-KLG (-C=O) to form a O-C-O interlinkage. In this study, we showed that the ¹³C chem. shift of the carbonyl changed from δ200 towards high field to about δ96 after forming the intramol. ring. Two-dimensional HMBC experiments revealed that 2-keto-L-gulonic acid formed five-membered ring 2,5-hemiacetal derivatives in seven solvents, instead of six-membered 2,6-hemiacetal derivatives

Bopuxue Zazhi published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Formula: C6H10O7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Gao, Ming published the artcileOptimization of fermentation conditions for two-step fermentation of vitamin C, Synthetic Route of 526-98-7, the publication is Shipin Gongye Keji (2012), 33(14), 235-238, database is CAplus.

The conversion process from L-sorbose to 2-keto-L-gulonic acid in vitamin C two-step fermentation was mixed culture fermentation by acid-producing strain (Gluconobacter oxydans) and companion bacterium (Bacillus subtilis). In order to promote the coordination between the two strains and make it reach the best state, the nutrient condition and the culture conditions were researched by orthogonal test and single factor experiment The result showed that the optimal conditions were as follows: 8% L-sorbose, 1.4% corn steep liquor, 1.2% urea, 0.5% CaCO₃, 0.02% MgSO₄, liquid volume in 100 mL flask with 10 mL, culture temperature 29°C, inoculation amount 15%. The yield of 2-KGA increased by 2.45% under the optimized culture medium conditions compared with the original medium. It reached the fermentation end after 60 h which was shortened by 16 h than before and the fermentation efficiency improved by 0.29 g/L·h.

Shipin Gongye Keji published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Synthetic Route of 526-98-7.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Simon-Manso, Yamil published the artcileMetabolite Profiling of a NIST Standard Reference Material for Human Plasma (SRM 1950): GC-MS, LC-MS, NMR, and Clinical Laboratory Analyses, Libraries, and Web-Based Resources, Application of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, the publication is Analytical Chemistry (Washington, DC, United States) (2013), 85(24), 11725-11731, database is CAplus and MEDLINE.

Recent progress in metabolomics and the development of increasingly sensitive anal. techniques have renewed interest in global profiling, i.e., semiquant. monitoring of all chem. constituents of biol. fluids. In this work, we have performed global profiling of NIST SRM 1950, “Metabolites in Human Plasma”, using GC-MS, LC-MS, and NMR. Metabolome coverage, difficulties, and reproducibility of the experiments on each platform are discussed. A total of 353 metabolites have been identified in this material. GC-MS provides 65 unique identifications, and most of the identifications from NMR overlap with the LC-MS identifications, except for some small sugars that are not directly found by LC-MS. Also, repeatability and intermediate precision analyses show that the SRM 1950 profiling is reproducible enough to consider this material as a good choice to distinguish between anal. and biol. variability. Clin. laboratory data shows that most results are within the reference ranges for each assay. Inhouse computational tools have been developed or modified for MS data processing and interactive web display. All data and programs are freely available online at http://peptide.nist.gov/ and http://srmd.nist.gov/.

Analytical Chemistry (Washington, DC, United States) published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H7BClNO3, Application of (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts

Llorente, Briardo published the artcileDownregulation of polyphenol oxidase in potato tubers redirects phenylpropanoid metabolism enhancing chlorogenate content and late blight resistance, Related Products of alcohols-buliding-blocks, the publication is Molecular Breeding (2014), 34(4), 2049-2063, database is CAplus.

Land plants synthesize phenolic compounds involved in plant defense against invading pathogens through the phenylpropanoid pathway. Although not considered as part of the phenylpropanoid pathway, plant polyphenol oxidases (PPOs) are enzymes that catalyze cresolase and catecholase reactions on several phenolic compounds Here, transgenic potato (Solanum tuberosum) tubers with downregulated PPO genes (-PPO) were challenged with the oomycete pathogen Phytophthora infestans to investigate the interactions between PPO, phenylpropanoid metabolism, and disease resistance. We found that pathogen invasiveness was reduced in -PPO lines, while microscopic evidences suggested that the mechanism underlying the defense response involved the participation of phenolic compounds Detailed metabolite-profiling analyses demonstrated that the concentration of metabolites related to the phenylpropanoid pathway and chlorogenate in particular was largely altered in PPO-downregulated tubers. Silencing of PPO caused a shift in metabolism from phenylpropanoid precursors to downstream phenylpropanoid products. The presented results suggest that downregulation of PPO redirects the phenylpropanoid metabolism leading to the accumulation of defensive phenolic compounds in the plant cells, consequently enhancing resistance to the pathogen. These results emphasize the importance of components acting in parallel to canonical metabolic pathway constituents in influencing plant metabolism and reveal new scenarios for modulating the levels of phenolics in crops.

Molecular Breeding published new progress about 526-98-7. 526-98-7 belongs to alcohols-buliding-blocks, auxiliary class Sugar Units,Other Sugar Units, name is (3S,4R,5S)-3,4,5,6-Tetrahydroxy-2-oxohexanoic acid, and the molecular formula is C6H10O7, Related Products of alcohols-buliding-blocks.

Referemce:
https://en.wikipedia.org/wiki/Alcohol,
Alcohols – Chemistry LibreTexts