Author: tianli

Azman, Muhammad Imran published the artcileFabrication of poly(pentaerythritol tetrakis (3-mercaptopropionate)/dipentaerythritol penta-/hexa-acrylate)HIPEs macroporous scaffold with alpha hydroxyapatite via photopolymerization for fibroblast regeneration, HPLC of Formula: 7575-23-7, the main research area is polyHIPE macroporosity scaffold hydroxyapatite photopolymerization fibroblast regeneration.

Synthetic biomaterials that can be structured into porous scaffolds for support cell growth have played a role in developing the field of tissue engineering. This research focused on combination of biodegradable emulsion template along with the assisting of low-cost polymerization reaction. The appendage of ester-based surfactant, Hypermer B246, played a vital role which gave an outstanding dispersion in HIPEs system and degradability. PolyHIPEs were prepared by using domestic UV light source for producing a multiscale porosity material. The morphol. showed a promising result of poly(pentaerythritol tetrakis (3-mercaptopropionate)/dipentaerythritol penta-/hexa-acrylate)HIPEs with varied Hypermer B246 surfactant concentration resulting in the pores size increased in between 51.2 ± 9.8μm to 131.4 ± 26.32μm. Cellular moieties of poly(TT/DPEHA) HIPEs were confirmed by using SEM while inclusion of hydroxyapatite were confirmed by SEM, FTIR and EDX-SEM and quantified by thermogravimetric anal. The maximum stress and compressive modulus of the obtained materials were significantly enhanced with HA up to five percent by weight Poly(TT/DPEHA)HIPEs with HA showed the ability for the cell attachment and the adhesion/proliferation of the cells, suggested that poly(TT/DPEHA) HIPEs with HA were suitable for biomaterial application.

Crystals published new progress about Cell adhesion. 7575-23-7 belongs to class alcohols-buliding-blocks, name is Pentaerythritol tetra(3-mercaptopropionate), and the molecular formula is C17H28O8S4, HPLC of Formula: 7575-23-7.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Yu, Peng published the artcileTemperature dependence of mitoflash biogenesis in cardiac mitochondria, Related Products of alcohols-buliding-blocks, the main research area is mitochondrial mitoflash cardiomyocyte temperature.

Mitochondrial flashes represent fundamental biochem. and biophys. dynamics of the organelle, involving sudden depolarization of mitochondrial membrane potential (ΔΨm), bursting production of ROS, and accelerated extrusion of matrix protons. Here we investigated temperature dependence of mitoflash biogenesis as well as ΔΨm oscillations, a subset of which overlapping with mitoflashes, in both cardiac myocytes and cardiac mitochondria. Unexpectedly, we found that mitoflash biogenesis was essentially temperature-independent in intact cardiac myocytes, evidenced by the constancy of frequency as well as amplitude and rise speed over 5°C-40°C. Moderate temperature dependence was found in single mitochondria charged by respiratory substrates, where mitoflash frequency was decreased over 5°C-20°C with Q10 of 0.74 for Complex I substrates and 0.83 for Complex II substrate. In contrast, ΔΨm oscillation frequency displayed a neg. temperature dependence at 5°C-20°C with Q10 of 0.82 in intact cells, but a pos. temperature dependence at 25°C – 40°C with Q10 of 1.62 in isolated mitochondria charged with either Complex I or Complex II substrates. These results suggest a temperature compensation of mitoflash frequency at both the mitochondrial and extra-organelle levels, and underscore that mitoflashes and ΔΨm oscillations are related but distinctly different mitochondrial functional dynamics.

Archives of Biochemistry and Biophysics published new progress about Cardiomyocyte. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Related Products of alcohols-buliding-blocks.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Colin, Camille published the artcileSingle-particle tracking method in fluorescence microscopy to monitor bioenergetic responses of individual mitochondria, Related Products of alcohols-buliding-blocks, the main research area is single particle tracking mitochondrial permeability transition pore fluorescence microscopy; Bioenergetics; Fiji software; Fluorescence microscopy; Membrane potential; Mitochondria; Single organelle; Single particle tracking; TrackMate.

The spectroscopic methods commonly used to study mitochondria bioenergetics do not show the diversity of responses within a population of mitochondria (isolated or in a cell), and/or cannot measure individual dynamics. New methodol. developments are necessary in order to improve quant. and kinetic resolutions and eventually gain further insights on individual mitochondrial responses, such as studying activities of the mitochondrial permeability transition pore (mPTP). The work reported herein is devoted to study responses of single mitochondria within a large population after isolation from cardiomyocytes. Mitochondria were preloaded with a commonly used membrane potential sensitive dye (TMRM), they are then deposited on a plasma-treated glass coverslip and subsequently energized or inhibited by additions of usual bioenergetics effectors. Responses were analyzed by fluorescence microscopy over few thousands of mitochondria simultaneously with a single organelle resolution We report an automatic method to analyze each image of time-lapse stacks based on the TrackMate-ImageJ plug-in and specially made Python scripts. Images are processed to eliminate defects of illumination inhomogeneity, improving by at least two orders of magnitude the signal/noise ratio. This method enables us to follow the track of each mitochondrion within the observed field and monitor its fluorescence changes, with a time resolution of 400 ms, uninterrupted over the course of the experiment Such methodol. improvement is a prerequisite to further study the role of mPTP in single mitochondria during calcium transient loading.

Methods in Molecular Biology (New York, NY, United States) published new progress about Cardiomyocyte. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Related Products of alcohols-buliding-blocks.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Wang, Wenying published the artcileHemichannel-mediated volume regulation contributes to IPC-induced cardiomyocyte protection, Related Products of alcohols-buliding-blocks, the main research area is IPC cardiomyocyte protection hemichannel mediated volume regulation contribute; capacity regulation; hemichannels; ischemic preconditioning; myocardial protection.

Cx43 has been documented to be involved in ischemic preconditioning (IPC). However, the participation of Cx43-formed hemichannels in IPC and the potential underlying mechanisms remain unclear. The present study focused on cardiomyocytes’ volume regulation during IPC to investigate the role of hemichannels in the IPC-induced cardioprotection. In the study, mice cardiomyocytes were resp. treated with a hemichannel blocker, octanol or 18a-Glycyrrhizic acid (18a-GA), and a Cx43-silenced lentivirus. They were subsequently cultured in hypotonic solution to simulate ischemic reperfusion (SIR) and systemic ischemic preconditioning (SIP). Cell morphol. and volumetric (area) change were detected by inverted microscopy at 30 min following the addition of hypotonic solution Cardiomyocyte mortality was assessed by trypan blue stain assay. The analyses revealed that regardless of the treatments, hypotonic solution aggravated cell edema: Compared with the initial condition (the moment before the solution addition, 0 min), the volumetric area increased significantly 30 min later (for hypotonic + DMSO, 5,050 ± 1,511 vs. 3,464 ± 723μm2; for hypotonic+scramble lentiviral vector, 5,517 ± 1,128 vs. 2,331 ± 536μm2; P<0.05, resp.). Either treatment alleviated the edematous condition when a comparison was made between 30 min after the hypotonic addition and 0 min (for hypotonic+octanol, 2,990 ± 765 vs. 2,821 ± 773μm2; for hypotonic+18a-GA, 4,817 ± 1,306 vs. 4,762 ± 1,271μm2; for hypotonic + Cx43-silenced, 3,627 ± 688 vs. 3,419 ± 814μm2; P>0.05 for all). Notably, results indicated that the SIP group had lower mortality rates compared with its SIR counterpart; the hypotonic + octanol, hypotonic + 18a-GA, and hypotonic + Cx43-silenced group showed markedly-declined mortality when compared with their resp. control groups (resp., 35.70 ± 1.02, 30.76 ± 2.20 vs. 53.58 ± 2.14%; 30.89 ± 2.37 vs. 54.12 ± 2.55%; P<0.05 for all). The results suggest that ischemic preconditioning may provide cardioprotection by blocking the opening of the hemichannels and further mediating the volume regulation of cardiomyocytes. Experimental and Therapeutic Medicine published new progress about Cardiomyocyte. 111-87-5 belongs to class alcohols-buliding-blocks, name is n-Octanol, and the molecular formula is C8H18O, Related Products of alcohols-buliding-blocks.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

DiMario, Robert J. published the artcileA single serine to alanine substitution decreases bicarbonate affinity of phosphoenolpyruvate carboxylase in C4Flaveria trinervia, Safety of (S)-2-hydroxysuccinic acid, the main research area is Flaveria phosphoenolpyruvate carboxylase bicarbonate affinity substitution.

Phosphoenolpyruvate (PEP) carboxylase (PEPc) catalyzes the first committed step of C4 photosynthesis generating oxaloacetate from bicarbonate (HCO3-) and PEP. It is hypothesized that PEPc affinity for HCO3- has undergone selective pressure for a lower KHCO3 (Km for HCO3-) to increase the carbon flux entering the C4 cycle, particularly during conditions that limit CO2 availability. However, the decrease in KHCO3 has been hypothesized to cause an unavoidable increase in KPEP (Km for PEP). Therefore, the amino acid residue S774 in the C4 enzyme, which has been shown to increase KPEP, should lead to a decrease in KHCO3. Several studies reported the effect S774 has on KPEP; however, the influence of this amino acid substitution on KHCO3 has not been tested. To test these hypotheses, membrane-inlet mass spectrometry (MIMS) was used to measure the KHCO3 of the photosynthetic PEPc from the C4Flaveria trinervia and the non-photosynthetic PEPc from the C3 F. pringlei. The cDNAs for these enzymes were overexpressed and purified from the PEPc-less PCR1 Escherichia coli strain. Our work in comparison with previous reports suggests that KHCO3 and KPEP are linked by specific amino acids, such as S774; however, these kinetic parameters respond differently to the tested allosteric regulators, malate and glucose-6-phosphate.

Journal of Experimental Botany published new progress about Carboxylation. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Safety of (S)-2-hydroxysuccinic acid.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Zhang, Yao published the artcileIDH2 compensates for IDH1 mutation to maintain cell survival under hypoxic conditions in IDH1-mutant tumor cells, Category: alcohols-buliding-blocks, the main research area is mutation cell survival proliferation glioma colon cancer.

Mutations of isocitrate dehydrogenase (IDH) 1 and 2 occur in low-grade gliomas, acute myeloid leukemias and other types of solid cancer. By catalyzing the reversible conversion between isocitrate and α-ketoglutarate (α-KG), IDH1 and 2 contribute to the central process of metabolism, including oxidative and reductive metabolism IDH1 and 2 mutations result in the loss of normal catalytic function and acquire neomorphic activity, facilitating the conversion of α-KG into an oncometabolite, (R)-2-hydroxyglutarate, which can cause epigenetic modifications and tumorigenesis. Small-mol. inhibitors of mutant IDH1 and 2 have been developed, and ongoing clin. trials have shown promising results in hematol. malignancies, but not in gliomas. These previous findings make it necessary to identify the mechanism and develop more effective therapies for IDH1-mutant gliomas. In the present study, it was demonstrated that under hypoxic conditions, patient-derived primary glioma cells and HCT116 cells, both of which carry a monoallelic IDH1 arginine 132 to histidine mutation (R132H), have a slower growth rate than the corresponding wild-type IDH1 cells. Western blot anal. showed that IDH1 R132H-mutant cancer cells exhibited upregulated IDH2 protein expression under hypoxic conditions. Furthermore, the silencing of IDH2 using small interfering RNA significantly inhibited the growth of IDH1-mutant cells under hypoxic conditions. Finally, [U-13C5]glutamine tracer anal. showed that IDH2 knockdown reduced the reductive carboxylation of α-KG into isocitrate in HCT116R132H/+ cells under hypoxic conditions. The present study showed for the first time, to the best of our knowledge, that IDH2 plays a compensatory role in maintaining reductive carboxylation-dependent lipogenesis and proliferation in IDH1 R132H tumor cells. Therefore, IDH2 could serve as a potential anti-tumor target for IDH1-mutant tumors, which may provide a new strategy for treatment.

Molecular Medicine Reports published new progress about Carboxylation. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, Category: alcohols-buliding-blocks.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Ribeiro, Rita published the artcileComparing the effect of Thymus spp. essential oils on Candida auris, Product Details of C10H18O, the main research area is Thymus Candida auris essential oil comparing effect.

Candida auris is an emergent yeast pathogen responsible for many invasive fungal infections due to its multidrug-resistant character and its huge transmission ability. Essential oils (EOs) obtained from several aromatic plants have been regarded as an alternative treatment upon to fungal infections. For example, Thymus spp. are known by their antifungal effect due to the presence of some volatile compounds in their EOs, such as carvacrol, thymol, linalool and gamma-terpinene. So, the main goal of this work was to compare the effect of several EOs from Thymus spp. on C. auris biofilm. The antifungal activity of Thymus vulgaris, Thymus zygis, Thymus satureioides and Thymus mastichina against planktonic cells of C. auris NCPF 8971 was assessed by agar disk diffusion method. The effect of these EOs with direct or vapor phases on preformed biofilms was evaluated by colony-forming unitsprime enumeration. Importantly it was noticed a completely different range of action between the EOs from the same genus. While T. vulgaris showed the biggest antifungal effect with a halo of 59.75 plusmn 15.75 mm, T. mastichina for the other side, presented a halo of 13.13 plusmn 1.36 mm, showing a very low activity. In addition, the direct application of T. vulgaris and T. zygis EOs demonstrated higher antifungal activity against C. auris biofilms than vapor phase application. However, T. vulgaris also showed significant action in the vapor phase mode. The chem. composition of Thymus EOs was analyzed by GC and GC-MS, and was demonstrated that they have different chem. profiles, namely in the amount of phenolic compounds, which justify the different antifungal actions.In conclusion, T. vulgaris and T. zygis oils, can be pointed out as a great contribution to the treatment of C. auris infections, being promising alternatives to conventional therapy. However, the efficiency of the EOs differs substantially between the four species of Thymus. Therefore, the selection of natural therapies should always have in account EOs composition

Industrial Crops and Products published new progress about Candida auris. 124-76-5 belongs to class alcohols-buliding-blocks, name is rel-(1R,2R,4R)-1,7,7-Trimethylbicyclo[2.2.1]heptan-2-ol, and the molecular formula is C10H18O, Product Details of C10H18O.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Hu, Guilin published the artcileEffect of roasting degree of coffee beans on sensory evaluation: Research from the perspective of major chemical ingredients, COA of Formula: C4H6O5, the main research area is roasting coffee beans; Chemical ingredients; Cluster heatmap; Multivariate analysis; Roasted coffee beans; Sensory indicator; Sensory molecular network.

As the most consumed beverage in the world, the material basis of the sensory quality for roasted coffee beans has always received much attention. The objective of the present study was to clarify the phys. morphol. changes, main chem. ingredients and cupping scores of arabica coffee beans of different roasting degrees, by SEM (SEM), NMR (NMR) and sensory anal., resp. Statistical anal. of the data by multivariate anal. demonstrated that trigonelline, sugars, malate, quinic acids, γ-butyro-lactone and acetate have the potential to be new roasting markers. Addnl., in all the sensory indicators, body and acidity were found to be susceptible to roasting degree. Basing on cluster heatmap and sensory mol. network, the complex relationships between sensory indicators and ingredients were discussed. The results of partial least squares regression (PLSR) showed that the content of the main coffee ingredients can be used to predict the body score.

Food Chemistry published new progress about Boiling point. 97-67-6 belongs to class alcohols-buliding-blocks, name is (S)-2-hydroxysuccinic acid, and the molecular formula is C4H6O5, COA of Formula: C4H6O5.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Wu, Haijun published the artcileEthanol-Assisted Hydrothermal Liquefaction of Poplar Using Fe-Co/Al2O3 as Catalyst, Related Products of alcohols-buliding-blocks, the main research area is ethanol hydrothermal liquefaction iron cobalt alumina catalyst.

Although the conversion of lignocellulosic biomass into bio-oil with high yield/quality through hydrothermal liquefaction (HTL) is promising, it still faces many challenges. In this study, a Fex-Co(1-x)/Al2O3 catalyst was prepared with the coprecipitation method and low-content ethanol was used as the cosolvent for the HTL of poplar. The results showed that the Fex-Co(1-x)/Al2O3 catalyst significantly promoted the yield and energy recovery rate (ERR) of bio-oil compared with the control (10% ethanol content). At 260 °C for 30 min, 60Fe-40Co/Al2O3 had the best catalytic effect, achieving the highest bio-oil yield (67.35%) and ERR (93.07%). As a multifunctional bimetallic catalyst, Fex-Co(1-x)/Al2O3 could not only increase the degree of hydrogenation deoxidization of the product but also promote the diversity of phenolic compounds gained from lignin. The bio-oil obtained from HTL with Fex-Co(1-x)/Al2O3 as catalyst contained lower heterocyclic nitrogen, promoting the transfer of more bio-oil components to substances with lower b.p.

Energies (Basel, Switzerland) published new progress about Boiling point. 584-02-1 belongs to class alcohols-buliding-blocks, name is 3-Pentanol, and the molecular formula is C5H12O, Related Products of alcohols-buliding-blocks.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts

Yang, Haihong published the artcileDegradation models, structure, rheological properties and protective effects on erythrocyte hemolysis of the polysaccharides from Ribes nigrum L, Related Products of alcohols-buliding-blocks, the main research area is Ribes nigrum polysaccharide structure rheol erythrocyte hemolysis protective effect; Blackcurrant polysaccharide; Degradation model; Erythrocyte hemolysis; Rheological properties; Structure; Ultrasonic treatment.

The polysaccharides from blackcurrant (Ribes nigrum L.) fruits were degraded by ultrasonic irradiation Results showed that viscosity-average mol. weight decreased with increasing ultrasonic time or power. The degradation was fitted to the second-order kinetics model and midpoint chain scission model. Gas chromatog. anal. demonstrated that the native polysaccharide and three degraded polysaccharides were composed of the same monosaccharides but in different ratios. Fourier transform IR and NMR spectroscopic analyses revealed the presence of α-, β-pyranose rings and the same six sugar residues in the four blackcurrant polysaccharides. Compared to the native polysaccharide, three degraded polysaccharides displayed better rheol. properties and stronger protective effects against erythrocyte hemolysis. Collectively, the results support the potential utility of blackcurrant polysaccharides as natural antioxidants.

International Journal of Biological Macromolecules published new progress about Black currant. 59-23-4 belongs to class alcohols-buliding-blocks, name is (2R,3S,4S,5R)-2,3,4,5,6-Pentahydroxyhexanal, and the molecular formula is C6H12O6, Related Products of alcohols-buliding-blocks.

Referemce:
Alcohol – Wikipedia,
Alcohols – Chemistry LibreTexts